AIM: Platycodin D (PD), an oleanane kind of triterpenoid saponin, possesses various pharmacological activities. We aimed to investigate the effects of PD in pulmonary fi brosis. METHOD: MRC-5 cells were induced by transforming growth factor-beta1 (TGF-β1) to simulate the pulmonary fi brosis in vitro. Cell viability was determined using a CCK-8 kit in the absence or presence of PD. Then, the expression of proliferation-related proteins was detected using immunofl uorescence assay or western blot analysis. Moreover, the levels of infl ammatory factors were examined. Subsequently, the ability of cell migration was evaluated using wound healing assay. Additionally, western blot analysis was employed to determine migration-and extracellular matrix accumulation (ECM)-related proteins expression. RESULTS: Results indicated that PD exposure signifi cantly dose-dependently inhibited TGF-β1 induced proliferation in MRC-5 cells. Additionally, the contents of infl ammatory factors were notably inhibited with PD treatment. Furthermore, signifi cant decrease in migration of TGF-β1-stimulated MRC-5 cells was observed after PD intervention. Afterwards, PD remarkably suppressed the expression of alpha smooth muscle actin (α-SMA), collagen I (Col I), collagen III (Col III) and E-cadherin (E-cad). CONCLUSIONS: PD attenuated proliferation and ECM accumulation in TGF-β1 induced lung fi broblasts, providing experimental support for the clinical application of PD in the treatment of pulmonary fi brosis (Fig. 6, Ref. 33).
ObjectiveThe prognostic nutritional index (PNI) is an important prognostic factor for survival outcomes in various hematological malignancies. The current study focused on exploring the predictive value of the PNI in newly diagnosed follicular lymphoma (FL) in China.Materials and methodsThe clinical indicators and follow-up data of 176 patients who received chemotherapy or immunotherapy combined with chemotherapy with FL in our hospital from January 2016 to March 2022 were retrospectively analyzed. Cox proportional hazard model was used for univariate and multivariate analyses. Kaplan–Meier curves were used to calculate survival rates and draw survival curves. The log-rank test was applied to compare differences between groups.ResultsThe optimal cut-off value of PNI was 44.3. All patients were divided into a high PNI group (>44.3) and a low PNI group (≤44.3). The low PNI group had a low CR rate and a high risk of death, with a tendency toward POD24, and Both OS and PFS were worse than those in the high PNI group. PNI was able to predict OS and PFS in FL patients and was the only independent predictor of OS (P = 0.014 HR 5.024; 95%CI 1.388∼18.178) in multivariate analysis. PNI could re-stratify patients into groups of high FLIPI score, high FLIPI2 score, no POD24, and rituximab combined with chemotherapy. Moreover, integrating PNI into the FLIPI and FLIPI2 models improved the area under the curve (AUC) for more accurate survival prediction and prognosis.ConclusionPNI is a significant prognostic indicator for newly diagnosed FL in China that can early identify patients with poor prognosis and guide clinical treatment decisions.
Castleman disease (CD) is a rare lymphoproliferative disorder. The mechanistic target of rapamycin (mTOR) pathway is a key regulator of various cellular functions, which may be related with the potential mechanisms of CD occurrence. We retrospectively collected the clinical information of 60 CD patients diagnosed in the First Affiliated Hospital of Zhengzhou University. And FFPE biopsy specimens were collected from 31 patients (12 unicentric CD patients and 19 multicentric CD patients) to detect the mTOR pathway protein expression. We are the first to demonstrate that thrombocytopenia and hypoalbuminemia are independent poor prognostic factors for CD. Moreover, mTOR activation was higher in CD compared to reactive lymphoid hyperplasia (used as a control group). This study offers some elucidation for the management and treatment of CD patients.
Epigenetic regulation plays a crucial part in the oncogenesis and treatment of diffuse large B-cell lymphoma (DLBCL). The H3K9me3-specific histone methyltransferase SUV39H1 is a significantly epigenetic gene that promotes the progression of a variety of malignancies. However, the roles of SUV39H1 in DLBCL remain unclear. By retrieving Oncomine, GEPIA, CCLE, UALCAN, and TCGA databases, we observed that the expression of SUV39H1 was higher in DLBCL tissues than in normal and other cancer tissues. Combined with immunohistochemical validation assay, we analysed clinical characteristics of DLBCL patients. The results showed that high expression of SUV39H1 was closely associated with age over 50 years old (P=0.014) and low albumin level (P=0.015). In the prognostic analyses, DLBCL patients in GEPIA database showed that the high SUV39H1 expression group had a lower disease-free survival (DFS) rate than the low SUV39H1 expression group (P=0.035). Finally, we discovered that expressions of CD86+ and CD163+ macrophages have high correlations with SUV39H1+ in DLBCL tissues(with P=0.037 and P=0.045,respectively). SUV39H1-associated macrophages may downregulate T lymphocyte subsets, especially Treg cells in DLBCL(P=0.003). In summary, SUV39H1 might be not only a potential target for the epigenetic therapy and immunotherapy of DLBCL, but also a clinical indicator for doctors to evaluate the trend of disease development.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.