Chromium toxicity is considered as a major problem for agricultural soil that reduced crop productivity by affecting photosynthetic tissues. Exogenous application of melatonin can alleviate the adverse effects of chromium toxicity on plant growth. However, little is known about its effect on thylakoidal protein complexes responsible for conversion of solar energy to biochemical energy. Chlorophyll fluorescence a transients considered one of the best non-invasive and rapid method for the evaluation of photosynthetic (Photosystem II) efficiency of plants and plant health under environmental stress conditions. In the present study, three-week old plants of two canola cultivars AC-Excel and DGL were applied to melatonin (0, 1, 5, 10 μM) when grown under chromium stress (0, 50 and 100 μM) for further two weeks. Chromium stress reduced the growth (fresh and dry weights of shoots and roots) of both canola cultivars and exogenous application of 5 and 10 μM melatonin improved the growth of canola at 50 or 100 μM chromium stress. This improvement was greater in cv DGL than in AC-Excel. Increasing chromium decreased the photosynthetic pigments (chlorophyll a and chlorophyll b ). However, 5 and 10 μM melatonin application improved chlorophyll a at 50 μM chromium stress. Structural stability and efficiency of photosystem II (PSII) measured as performance index (PI ABS ) and ratios of fluorescence (Fv/Fm, Fv/Fo) Fv decreased due to chromium stress. JIP-test parameters showed that chromium stress increased the absorption and trapping fluxes with decrease in electron transport fluxes which caused the damage to reaction centers (RC), detachment of oxygen evolving complex (OEC) from RC or inefficiency of electron transfer from OEC to RC. Such adverse effects were greater in cv AC-Excel. However exogenous application of melatonin improved PI ABS , electron transport per reaction center (ET/RC), reduced variable fluorescence at J step (V J ) reflecting melatonin protected PSII from chromium stress induced damage by protecting OEC. Thus, OJIP fluorescence transients are quite helpful for understanding the intersystem electron transport beyond photosystem II in canola cultivars due to melatonin application under chromium stress. Findings Exogenous application of melatonin alleviated toxic effects of chromium on plant growth of canola by modulating photosynthesis, enhanced photosystem II efficiency and regulation of electron transport flux to protect photo-inhibition of PSII from oxidative damage.

Developing drought-tolerant cultivars is mainly restricted due to poor knowledge of the mechanism behind drought tolerance. In the present work, available germplasm of Vigna radiata (mung bean) was screened for drought tolerance using multiple agronomic and physiological parameters and used to selected one drought-tolerant (NM-13-1) and one drought-sensitive (NM-54) cultivar for further studies. Plant water status and PSII activity were found to be potential physiological discriminating traits. Changes in PSII and PSI activity, accumulation of proline, oxidative damage, and antioxidants were further assessed in selected drought-sensitive and droughttolerant cultivars. Drought stress reduced PSII efficiency and electron transport in both mung bean cultivars. Drought increased NPQ and Y(NPQ), a greater increase in NPQ and Y(NPQ) was found in the drought-tolerant cv NM-13-1, indicating that the drought-tolerant cultivar managed over-excitation of PSII by safe heat dissipation via photo-protective component of NPQ. A decrease in PSI efficiency with an increase in donor end limitation of PSI in both mung bean cultivars further confirmed that the electron transport through PSII became down-regulated. However, the droughtsensitive cv. NM-54 had poor ability to manage over-excitation of PSII through buildup of Y(NPQ) thereby causing greater oxidative stress. Mung bean cultivars counteracted oxidative stress by accumulation of proline and increasing POD activities. Drought-tolerant cv. NM-13-1 had higher proline accumulation and antioxidant potential than in the drought-sensitive cultivar. Overall, drought tolerance in the mung bean cultivars can be related to plant water status, PSII activity, Y(NPQ), and POD activity, which can be effectively used for selecting mung bean cultivars for drought tolerance.

In the present study, the mung bean cv. NM-13-1 Tol was selected as drought-tolerant and NM-54 Sens as drought-sensitive. The effects of progressive drought (16 days) on the photosystem II (PSII) activity was assessed using OJIP and JIP-test in the selected two mung bean cultivars differing in drought tolerance. Drought stress reduced the relative water content to 70% (at threshold) and 62% (below the threshold) in cv. NM-13-1 Tol and NM-54 sens , respectively. The greater reduction in quantum yield of PSII in cv.NM-54 sens due to drought stress was due to PSII photodamage. Raw OJIP induction curves and F o and F m normalised curves showed that significant changes in fluorescence occurred at the O, J, I and P steps only in cv. NM-54 sens . Double normalised differential kinetics indicated adverse effects at the antennae, oxygenevolving complex and intersystem electron acceptors in cv.NM54 sens . Moreover, JIPtest analysis showed that drought stress caused a greater decrease in performance index (PI ABS ) in cv.NM-54 sens as compared to that in cv. NM-13-1 Tol , which is associated with an increase in V j , rate of accumulation of closed reaction centres (M o ), energy fluxes for absorption (ABS/RC), trapping (TR o /RC), electron transport (ET o / RC), and dissipation of absorbed energy as heat (DI o /RC). In conclusion, two-week drought stress reduced the RWC below the threshold in cv.NM54 sens , which resulted in damages at the donor and acceptor sides of PSII. However, cv.NM-13-1 Tol somehow maintained the RWC around the threshold and thus protected PSII. Of various JIP-test parameters, PI ABS , F v /F m , V j and M o are key indicators of drought stress tolerance in mung bean cultivars.

Salt tolerant wheat cultivars may be used as genetic resource for wheat breeding to ensure yield stability in future. The study was aimed to select salt tolerant cultivar(s) to identify novel source of salt tolerance in local wheat germplasm. Initially, 40 local wheat cultivars were screened at 150 mM NaCl stress at seedling stage. Selected salt-tolerant (three; S-24, LU-26S and Pasban-90) and salt-sensitive (four; MH-97, Kohistan-97, Inqilab-91 and Iqbal-2000) wheat cultivars were further evaluated using growth, yield, biochemical and physiological attributes. Growth and yield of selected cultivars were reduced under salt stress due to decline in plant water status, limited uptake of macronutrients (N, P and K), reduced K+/Na+ ratio, photosynthetic pigments and quantum yield of PSII. Wheat plants tried to acclimate salt stress by osmotic adjustment (accumulation of total soluble sugars, proline and free amino acids). Degree of salinity tolerance in cvs. S-24 and LU-26S found to be associated with maintenance of K+/Na+ ratio, osmo-protectant and photosynthetic activity and can be used as donor for salt tolerance in wheat breeding program at least in Pakistan. These cultivars can be further characterized using molecular techniques to identify QTLs/genes for salt exclusion, osmo-protectant and photosynthetic activity for molecular breeding.