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The world is facing a global pandemic of COVID-19 caused by the SARS-CoV-2 coronavirus. Here we describe a collection of codon-optimized coding sequences for SARS-CoV-2 cloned into Gateway-compatible entry vectors, which enable rapid transfer into a variety of expression and tagging vectors. The collection is freely available. We hope that widespread availability of this SARS-CoV-2 resource will enable many subsequent molecular studies to better understand the viral life cycle and how to block it.

Highlights d CRISPR screen identifies SEMA6A and SEMA6B as receptors for P. sordellii lethal toxin TcsL d Soluble SEMA6A ectodomain protects mouse lungs from TcsL-induced edema d 3.3 Å cryo-EM structure of TcsL bound to SEMA6A reveals atomic details of the interaction d 15 mutations in the TcsL receptor-binding interface rewire receptor specificity

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