We have reported the photochemical regulation of the intracellular antisense effect of antisense probes containing ap hoto-responsive artificial nucleic acid, 3-cyanovinylcarbazole nucleoside ( CNV K). Here we focus on the importance of the photocrosslinking rate on the inhibitory effect on gene expression using photocrosslinkable antisense probes (pcASOs). The inhibitory effect of pcASOs on GFP gene expression was dependento nt he photocrosslinking rate of 3-cyanovinylcarbazole with d-threoninol ( CNV D), CNV K, or psoralen. The ultrafast RNA photocrosslinking induced the formation of at hermally irreversible covalent bond between pcASOs andt he target RNA. These ASOs strongly inhibited gene expression only when the photocrosslinking rate was faster than the random walk of branch migration. In addition, pcASOs containing CNV Do r CNV Kt argeted the RNAs with secondary structures.T hese results indicatet he regulatory effect of photocrosslinker and photoirradiation energy using pcASOs on the gene expression level.[a] Prof.
Ultrafast RNA photocrosslinking can regulate the intracellular gene expression via antisense effects using photocrosslinkable antisense oligodeoxynucleotides (pcASOs). The inhibitory effect of pcASOs on gene expression was dependent on the photocrosslinking rate of 3‐cyanovinylcarbazole with d‐threoninol (CNVD), CNVK, or psoralen. The ultrafast RNA photocrosslinking induced the formation of a thermally irreversible covalent bond between pcASOs and the target RNA. These results indicate a regulatory effect of the photocrosslinker and photoirradiation energy using pcASOs on the gene expression level. More information can be found in the Communication by Kenzo Fujimoto, Hung Yang‐Chun, and Shigetaka Nakamura on page 1912 in Issue 11, 2019 (DOI: 10.1002/asia.201801917).
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