Background: SsfX3 is an acyltransferase that can acylate tetracycline-like molecules at C-4. Results: Crystal structure showed SsfX3 contains a structural N-terminal -sandwich domain and a catalytic C-terminal hydrolase domain. Mutagenesis revealed both are essential for activity.
Conclusion:The N-terminal domain is recruited to bind the large "small molecule" substrate. Significance: New structural knowledge enables engineering of the acyltransferase toward synthesis of tetracycline analogs.
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