Background: Retinoblastoma (RB) is a common malignancy in children eyes. Aberrant microRNA (miR) expression is observed in many cancer cases. miR-515-5p is reported to be concerned with the course of many cancers. This study explores the role of miR-515-5p in proliferation and drug sensitivity of RB cells. Methods: Human RB cell lines (WERI-RB1, SO-RB50 and Y79) and human retinal pigment epithelial cell line ARPE-19 were utilized in this study. Drug-resistant cells SO-RB50/VCR and SO-RB50/CBP were constructed for the following experiments. The expressions of miR-515-5p and Notch1 in RB cells were detected. Notch1 was significantly upregulated in RB cells while miR-515-5p was notably downregulated. Then, the binding relationship between miR-515-5p and Notch1 was predicted and verified. Results: miR-515-5p negatively regulated Notch1 expression. In vitro and in vivo experiments revealed that overexpressed miR-515-5p inhibited RB cell proliferation and enhanced drug sensitivity. Functional rescue experiment suggested that miR-515-5p regulated RB cell proliferation and drug sensitivity via inhibiting Notch1 expression. Conclusion: It could be concluded that overexpressed miR-515-5p suppressed proliferation and drug resistance of RB cells by targeting Notch1 expression, indicating that miR-515-5p might constitute a promising therapy target for RB.
Two-dimensional direct numerical simulations of partitioned thermal convection are performed using the thermal lattice Boltzmann method for the Rayleigh number (Ra) of 109 and the Prandtl number (Pr) of 7.02 (water). The influence of the partition walls on the thermal boundary layer is mainly focused on. Moreover, to better describe the spatially nonuniform thermal boundary layer, the definition of the thermal boundary layer is extended. The numerical simulation results show that the gap length significantly affects the thermal boundary layer and Nusselt number (Nu). The gap length and partition wall thickness have a coupled effect on the thermal boundary layer and the heat flux. Based on the shape of the thermal boundary layer distribution, two different heat transfer models are identified at different gap lengths. This study provides a basis for improving the understanding of the effect of partitions on the thermal boundary layer in thermal convection.
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