30Qing Mao (Phone +86 135 9418 0020;Abstract: An excessive immune response contributes to SARS-CoV, MERS-CoV and SARS-CoV-2 pathogenesis and lethality, but the mechanism remains unclear. In this study, the N proteins of SARS-CoV, MERS-CoV and SARS-CoV-2 were found to bind to MASP-2, the key serine protease in the lectin pathway of complement activation, resulting in aberrant complement activation and aggravated inflammatory lung injury. Either blocking the N protein:MASP-2 5 interaction or suppressing complement activation can significantly alleviate N protein-induced complement hyper-activation and lung injury in vitro and in vivo. Complement hyper-activation was also observed in COVID-19 patients, and a promising suppressive effect was observed when the deteriorating patients were treated with anti-C5a monoclonal antibody. Complement suppression may represent a common therapeutic approach for pneumonia induced by these 10 highly pathogenic coronaviruses. Short Title: SARS-CoV N over-activates complement by MASP-2One Sentence Summary: The lectin pathway of complement activation is a promising target for 15 the treatment of highly pathogenic coronavirus induced pneumonia.All rights reserved. No reuse allowed without permission.(which was not certified by peer review) is the author/funder, who has granted medRxiv a license to display the preprint in perpetuity.
Stretchable optical waveguides can sense curvature, elongation, and force in a prosthetic hand.
Flying insects capable of navigating in highly cluttered natural environments can withstand inflight collisions because of the combination of their low inertia 1 and the resilience of their wings 2 , exoskeletons 1 , and muscles. Current insect-scale (<10 cm, <5 g) aerial robots 3-6 use rigid microscale actuators, which are typically fragile under external impact. Biomimetic artificial muscles 7-10 capable of large deformation offer a promising alternative for actuation because they can endure the stresses caused by such impacts. However, existing soft actuators 11-13 have not yet demonstrated sufficient power density for liftoff, and their actuation nonlinearity and limited bandwidth further create challenges for achieving closed-loop flight control. Here we develop the first heavier-than-air aerial robots powered by soft artificial muscles that demonstrate open-loop, passively stable ascending flight as well as closed-loop, hovering flight. The robots are driven by 100 mg, multilayered dielectric elastomer actuators (DEA) that have a resonant frequency and power density of 500 Hz and 600 W/kg, respectively. To increase actuator output mechanical power and to demonstrate flight control, we present strategies to overcome challenges unique to soft actuators, such as nonlinear transduction and dynamic buckling. These robots can sense, and withstand, collisions with surrounding obstacles, and can recover from in-flight collisions by exploiting material robustness and vehicle passive stability. We further perform a simultaneous flight with two micro-aerial-vehicles (MAV) in cluttered environments. These robots rely on offboard amplifiers and an external motion capture system to provide power to the DEAs and control flights. Our work demonstrates how soft actuators can achieve sufficient power density and bandwidth to enable controlled flight, illustrating the vast potential of developing next-generation agile soft robots. Soft robotics 14-16 is an emerging field aiming to develop versatile systems that can safely interact with humans and manipulate delicate objects in unstructured environments. A major challenge in building softactuated mobile robots involves developing muscle-like actuators that have high energy density, bandwidth, robustness, and lifetime. Previous studies have described soft actuators that can be actuated chemically 17 , pneumatically 18,19 , hydraulically 20 , thermally 21,22 , or electrically 7,23. Among these soft transducers, DEAs have shown a combination of muscle-like energy density and bandwidth 8 , enabling the development of biomimetic robots capable of terrestrial 11,24,25 and aquatic locomotion 26,27. However, while there is growing interest in developing heavier-than-air, soft-actuated aerial robots, existing soft robots 11-13 have been unable to achieve liftoff due to limited actuator power density (<200 W/kg), bandwidth (<20 Hz), and difficulties of integration with rigid robotic structures such as transmission and wings. To enable controlled hovering flight of a soft-actuated robot,...
Soft robotics represents a new set of technologies aimed at operating in natural environments and near the human body. To interact with their environment, soft robots require artificial muscles to actuate movement. These artificial muscles need to be as strong, fast, and robust as their natural counterparts. Dielectric elastomer actuators (DEAs) are promising soft transducers, but typically exhibit low output forces and low energy densities when used without rigid supports. Here, we report a soft composite DEA made of strain-stiffening elastomers and carbon nanotube electrodes, which demonstrates a peak energy density of 19.8 J/kg. The result is close to the upper limit for natural muscle (0.4–40 J/kg), making these DEAs the highest-performance electrically driven soft artificial muscles demonstrated to date. To obtain high forces and displacements, we used low-density, ultrathin carbon nanotube electrodes which can sustain applied electric fields upward of 100 V/μm without suffering from dielectric breakdown. Potential applications include prosthetics, surgical robots, and wearable devices, as well as soft robots capable of locomotion and manipulation in natural or human-centric environments.
The design and fabrication of a rolled dielectric elastomer actuator is described and the parametric dependence of the displacement and blocked force on the actuator geometry, elastomer layer thickness, voltage, and number of turns is analyzed. Combinations of different elastomers and carbon nanotube electrodes are investigated and optimized to meet performance characteristics appropriate to tactile display applications, namely operation up to 200 Hz with a combination of a 1 N blocked force and free displacement of 1 mm, all within a volume of less than 1 cm3. Lives in excess of 50 000 cycles have been obtained. Key to meeting these objectives is control of the multilayering fabrication process, the carbon nanotube electrode concentration, the selection of a soft elastomer with low viscous losses, and a proof‐testing procedure for enhancing life cycle reliability.
The detailed mechanical design of a digital mask projection stereolithgraphy system is described for the 3D printing of soft actuators. A commercially available, photopolymerizable elastomeric material is identified and characterized in its liquid and solid form using rheological and tensile testing. Its capabilities for use in directly printing high degree of freedom (DOF), soft actuators is assessed. An outcome is the ∼40% strain to failure of the printed elastomer structures. Using the resulting material properties, numerical simulations of pleated actuator architectures are analyzed to reduce stress concentration and increase actuation amplitudes. Antagonistic pairs of pleated actuators are then fabricated and tested for four-DOF, tentacle-like motion. These antagonistic pairs are shown to sweep through their full range of motion (∼180°) with a period of less than 70 ms.
Corona Virus Disease 2019 (COVID-19) is a recently emerged life-threatening disease caused by SARS-CoV-2. Real- time fluorescent PCR (RT-PCR) is the clinical standard for SARS-CoV-2 nucleic acid detection. To detect SARS-CoV-2 early and control the disease spreading on time, a faster and more convenient method for SARS-CoV-2 nucleic acid detecting, RT-LAMP method (reverse transcription loop-mediated isothermal amplification) was developed. RNA reverse transcription and nucleic acid amplification were performed in one step at 63 ℃ isothermal conditions, and the results can be obtained within 30 minutes. ORF1ab gene, E gene and N gene were detected at the same time. ORF1ab gene was very specific and N gene was very sensitivity, so they can guarantee both sensitivity and specificity for SARS-CoV-2. The sensitivity of RT-LAMP assay is similar to RT-PCR, and specificity was 99% as detecting 208 clinical specimens. The RT-LAMP assay reported here has the advantages of rapid amplification, simple operation, and easy detection, which is useful for the rapid and reliable clinical diagnosis of SARS-CoV-2.
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