A multifunctional sialyltransferase has been cloned from Pasteurella multocida strain P-1059 and expressed in E. coli as a truncated C-terminal His6-tagged recombinant protein (tPm0188Ph). Biochemical studies indicate that the obtained protein is (1) an alpha2,3-sialyltransferase (main function), (2) an alpha2,6-sialyltransferase, (3) an alpha2,3-sialidase, and (4) an alpha2,3-trans-sialidase. The recombinant tPm0188Ph is a powerful tool in the synthesis of structurally diverse sialoside libraries due to its relaxed substrate specificity, high solubility, high expression level, and multifunctionality.

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Remote sensing can provide near real-time and dynamic monitoring of drought. The drought severity index (DSI), based on the normalized difference vegetation index (NDVI) and evapotranspiration/potential evapotranspiration (ET/PET), has been used for drought monitoring. This study examined the relationship between the DSI and winter wheat yield for prefecture-level cities in five provinces of eastern China during 2001-2016. We first analyzed the spatial and temporal distribution of droughts in the study area. Then the correlation coefficient between drought-affected area and detrended yield of winter wheat was quantified and the impact of droughts of different intensities on winter wheat yield during different growth stages was investigated. The results show that incipient drought during the wintering period has no significant impact on the yield of winter wheat, while moderate drought in the same period can reduce yield. Drought affects winter wheat yield significantly during the flowering and filling stages. Droughts of higher intensity have more significant negative effects on the yield of winter wheat. Monitoring of droughts and irrigation is critical during these periods to ensure normal yield of winter wheat. This study has important practical implications for the planning of irrigation and food security.

Decision-making regarding energy determines carbon emissions and the severity of climate change. Energy literacy plays a crucial role because well-informed citizens can support the design and implementation of smart and forward-looking policies. Research has shown that people hold misconceptions about energy, and for young students these may persist into adulthood. Thus, this study is to understand the energy literacy of junior high school students in Taiwan and what their misconceptions are as well as why and how they hold these. Energy literacy scales (ELS) were developed and served as the basis for a survey of 1652 students in five regions of Taiwan, in which most indicators for knowledge were designed corresponding to common misconceptions in the scientific and social context of energy issues. Through analyzing the survey questions and survey results, interview questions were designed and 10 students were interviewed to identify their misconceptions. A "conceptual logic map" model was developed for demonstrating the sources and patterns of misconceptions and their linkages. Potential educational strategies were then proposed, showing the applicability of the model. The combination of concept-oriented energy literacy surveys, interviews, and the conceptual logic map was proven to be an effective design for misconception identification and treatment.

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