The dominant pattern in U.S. meat consumption over the past two decades has been a steady increase in per capita poultry consumption, largely at the expense of beef consumption. Our findings suggest that the major factor governing this pattern is structural change. Specifically, health information or trend was found to be significant in each of the four equations estimated in the Rotterdam system. Moreover, the health-information elasticities in general are larger in absolute value than price elasticities, which suggests that small percentage changes in health information have larger impacts on meat consumption than equivalently small percentage changes in relative prices. The estimated effects of generic advertising, in contrast, were found to be modest and fragile. Copyright 1997, Oxford University Press.

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BackgroundHuman cells release nano-sized vesicles called exosomes, containing mRNA, miRNA and specific proteins. Exosomes from one cell can be taken up by another cell, which is a recently discovered cell-to-cell communication mechanism. Also, exosomes can be taken up by different types of cancer cells, but the potential functional effects of mast cell exosomes on tumor cells remain unknown.Methods and resultsExosomes were isolated from the human mast cell line, HMC-1, and uptake of PKH67-labelled exosomes by the lung epithelial cell line, A549, was examined using flow cytometry and fluorescence microscopy. The RNA cargo of the exosomes was analyzed with a Bioanalyzer and absence or presence of the c-KIT mRNA was determined by RT-PCR. The cell proliferation was determined in a BrdU incorporation assay, and proteins in the KIT-SCF signaling pathway were detected by Western blot. Our result demonstrates that exosomes from mast cells can be taken up by lung cancer cells. Furthermore, HMC-1 exosomes contain and transfer KIT protein, but not the c-KIT mRNA to A549 cells and subsequently activate KIT-SCF signal transduction, which increase cyclin D1 expression and accelerate the proliferation in the human lung adenocarcinoma cells.ConclusionsOur results indicate that exosomes can transfer KIT as a protein to tumor cells, which can affect recipient cell signaling events through receptor-ligand interactions.