Organic
matter is important for controlling arsenic reduction and
release under anoxic conditions. Humic substances (HS) represent an
important fraction of natural organic matter, yet the manner in which
HS affect arsenic transformation in flooded paddy soil has not been
thoroughly elucidated. In this study, anaerobic microcosms were established
with arsenic-contaminated paddy soil and amended with three extracted
humic fractions (fulvic acid, FA; humic acid, HA; and humin, HM).
The HS substantially enhanced the extent of arsenic reduction and
release in the order FA > HA > HM. It was confirmed that microbially
reduced HS acted as an electron shuttle to promote arsenate reduction.
HS, particularly FA, provided labile carbon to stimulate microbial
activity and increase the relative abundances of Azoarcus, Anaeromyxobacter, and Pseudomonas, all of which may be involved in the
reduction of HS, Fe(III), and arsenate. HS also increased the abundance
of transcripts for an arsenate-respiring gene (arrA) and overall transcription in arsenate-respiring Geobacter spp. The increase in both abundances lagged
behind the increases in dissolved arsenate levels. These results help
to elucidate the pathways of arsenic reduction and release in the
presence of HS in flooded paddy soil.
Fe(III) oxyhydroxides play critical roles in arsenic immobilization due to their strong surface affinity for arsenic. However, the role of bacteria in Fe(II) oxidation and the subsequent immobilization of arsenic has not been thoroughly investigated to date, especially under the micro-oxic conditions present in soils and sediments where these microorganisms thrive. In the present study, we used gel-stabilized gradient systems to investigate arsenic immobilization during microaerophilic microbial Fe(II) oxidation and Fe(III) oxyhydroxide formation. The removal and immobilization of dissolved As(III) and As(V) proceeded via the formation of biogenic Fe(III) oxyhydroxides through microbial Fe(II) oxidation. After 30 days of incubation, the concentration of dissolved arsenic decreased from 600 to 4.8 μg L-1. When an Fe(III) oxyhydroxide formed in the presence of As(III), most of the arsenic ultimately was found as As(V), indicating that As(III) oxidation accompanied arsenic immobilization. The structure of the microbial community in As(III) incubations was highly differentiated with respect to the As(V)-bearing ending incubations. The As(III)containing incubations contained the arsenite oxidase gene, suggesting the potential for microbially mediated As(III) oxidation. The findings of the present study suggest that As(III) immobilization can occur in microoxic environments after microbial Fe(II) oxidation and biogenic Fe(III) oxyhydroxide formation via the direct microbial oxidation of As(III) to As(V). This study demonstrates that microbial Fe(II) and As(III) oxidation are important geochemical processes for arsenic immobilization in micro-oxic soils and sediments.
This study explored biostimulation mechanisms with an electron donor and a shuttle for accelerating pentachlorophenol (PCP) transformation in iron-rich soils. The results indicated that indigenous microbial communities are important for PCP transformation in soils. Biostimulation of indigenous microbial communities by the addition of lactate and anthraquinone-2,6-disulfonate (AQDS) led to the enhanced rates of PCP dechlorination by the dechlorinating-and ironreducing bacteria in soils. The electrochemical studies using cyclic voltammograms and microbial current measurements confirmed the high reduction potential and the large amount of electrons generated under biostimulation conditions, which were responsible for the higher rates of PCP transformation. After biostimulation treatments by the additions of lactate and/or AQDS during PCP dechlorination processes, microbial community analysis by the terminal restriction fragment length polymorphism (T-RFLP) method showed the abundance terminal restricted fragments (T-RFs), an indicator of bacterial abundance, which represents the dechlorinating-and iron-reducing bacteria, suggesting their critical roles in PCP dechlorination in soils.
A fermentative facultative anaerobe, strain HS01 isolated from subterranean sediment, was identified as Aeromonas hydrophila by 16S rRNA sequence analysis. The biotransformation of 1,1,1-trichloro-2,2-bis(4-chlorophenyl) ethane (DDT), 1,1-dichloro-2,2-bis(4-chlorophenyl) ethylene (DDD), and 1,1-dichloro-2,2-bis (4-chlorophenyl) ethane (DDE) by HS01 was investigated in the presence of goethite and anthraquinone-2,6-disulphonic disodium salt (AQDS). The results demonstrated that HS01 was capable of reducing DDTs, goethite and AQDS. And goethite can significantly enhance the reduction of DDT, DDD and DDE to some extent, while the addition of AQDS can further accelerate the reduction of Fe(III) and DDTs. The products of DDT transformation were identified as a large amount of dominant DDD, and small amounts of 1-chloro-2,2-bis-(p-chlorophenyl)ethane (DDMU), unsym-bis(p-chlorophenyl)-ethylene (DDNU), and 4,4'-dichlorobenzophenone (DBP). The results of cyclic voltammetry suggested that AQDS could increase the amounts of reactive biogenic Fe(II), resulting in the enhanced transformation of DDTs. This investigation gives some new insight in the fate of DDTs related to iron- and humic-reducing bacteria.
We studied the mechanisms of microbial transformation in functional bacteria on 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane (DDT) in two different field soils, Haiyan (HY) and Chenghai (CH). The results showed that microbial activities had a steady dechlorination effect on DDT and its metabolites (DDx). Adding lactate or glucose as carbon sources increased the amount of Desulfuromonas, Sedimentibacter, and Clostridium bacteria, which led to an increase in adsorbed Fe(II) and resulted in increased DDT transformation rates. The electron shuttle of anthraquinone-2,6-disulfonic disodium salt resulted in an increase in the negative potential of soil by mediating the electron transfer from the bacteria to the DDT. Moreover, the DDT-degrading bacteria in the CH soil were more abundant than those in the HY soil, which led to higher DDT transformation rates in the CH soil. The most stable compound of DDx was 1,1-dichloro-2,2-bis(p-chloro-phenyl)ethane, which also was the major dechlorination metabolite of DDT, and 1-chloro-2,2-bis-(p-chlorophenyl)ethane and 4,4'-dichlorobenzo-phenone were found to be the terminal metabolites in the anaerobic soils.
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