Electron microscopic examination of ultra-thin sections of Escherichia coli cells treated with various concentrations of chlorhexidine diacetate reveal two effects. In low drug concentrations many cells lose electron dense material leaving behind empty shells, in higher concentrations the appearance of the cytoplasm is significantly affected. These results are discussed in the light of biochemical findings under identical conditions.
Investigations of the effect of low concentrations of Fentichlor on the metabolic activities of Staphylococcus aurew and Escherkhk coli indicate that the bacteriostatic action of the drug results from a specific inhibition of metabolic reactions within the cell which are energy‐requiring. Bacteriostatic concentrations of Fentichlor inhibit energy dependent uptake of amino acids, assimilation of amino acids and glucose into cellular material and total protein synthesis. In contrast, energy‐independent uptake of amino acids and the uptake of glucose is little affected. These concentrations do not inhibit respiratory enzymes and selective inhibition of energy metabolism by Fentiohlor probably results from a disruption of the mechanism of energy coupling within the cell.
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