The aim of the present study was to evaluate the upshot of microencapsulation on the stability and viability of probiotics in carrier food (ice cream) and simulated gastrointestinal (GIT) conditions. Purposely, Lactobacillus casei was encapsulated with two different hydrocolloids, that is, calcium alginate (Ca‐ALG) and whey protein concentrate (WPC) by using encapsulator. The obtained microbeads were characterized in terms of encapsulation efficiency and morphological features. Afterward, the probiotics in free and encapsulated form were incorporated into ice cream. The product was subjected for physicochemical, microbiological, and sensory attributes over a storage period of 80 days. Microencapsulation with both hydrogels significantly (p < .05) improved the viability of probiotics in both carrier food and simulated GIT conditions.The initial viable count of probiotics encapsulated with Ca‐ALG and WPC was 9.54 and 9.52 log CFU/ml, respectively, that declined to 8.59 and 8.39 log CFU/ml, respectively, over period of 80 days of storage. While nonencapsulated/free cells declined from 9.44 to 6.41 log CFU/ml during same storage period. Likewise, during in vitro GIT assay, encapsulated probiotic with Ca‐ALG and WPC showed 0.95 and 1.13 log reduction, respectively. On other hand, free probiotics showed significant 3.03 log reduction. Overall, microencapsulated probiotic exhibited better survival as compared to free cells. Moreover, the amalgamation of encapsulated and free probiotics affected the physicochemical (decrease in pH and increase in viscosity) was and sensory parameters of ice cream during storage.
A snack is a product which is easy to consume and needs no preparation before consumption. It can be used to satisfy the hunger between meal times (Herman, Polivy, Pliner, & Vartanian, 2019). People have been consuming fried snack products for years due to attractive aroma, flavor combinations, and texture. However, owing to their certain health hazards, there is an increasing interest among people for consumption of safe, healthy, and nutritious food (Santeramo et al., 2018). This has led to an emphasis on the production of food free from extra calories, sugars, and fat. Only a small number of crunchy snacks that can be considered healthy are available in the market nowadays. An exciting alternative to currently popular snacks is dried apple snacks. The use of vegetables and fruits in every day diet has led an increase in health benefits such as fewer calories, more dietary fiber and fiber content, and numerous nutritive components that include minerals and vitamins (Higgs, Liu, Collins, & Thomas, 2019). In a
The present study was designed to explicate the effect of encapsulation on the stability of Bifidobacterium bifidum in pasteurized grape juice and in vitro gastrointestinal conditions. Purposely, hydrogel beads were prepared using sodium alginate and K‐carrageenan by internal gelation method. Free and encapsulated probiotics were incorporated in pasteurized grape juice samples. The initial count in grape juice samples with free probiotic cells was calculated as 9.35 log cfu/mL that reduced to 6.58 log cfu/mL after 35 days. Likewise, the probiotic count, that is, in samples containing encapsulated probiotics reduced to a level of 8.51 log cfu/mL and 7.09 log cfu/mL after the mentioned storage period. The number of free viable cells in juice samples was lower than (107 cfu/g) the minimum recommend level. Similarly, the free cells showed a poor survival under simulated gastrointestinal conditions. Furthermore, encapsulation affected the physicochemical (pH, acidity, and Brix) and sensorial characteristics of the juice samples. Practical applications The probiotic delivery through carrier food and under hostile gastrointestinal conditions is a great challenge due to their low survival. The encapsulation technology can be used to protect the target delivery of probiotics. The encapsulation technology is a useful tool for delivering the probiotics for both dairy and nondairy food matrices. Encapsulation also ensures the recommended therapeutic level (1107–108 cfu) during transit and storage.
In the current study, Lactobacillus acidophilus was encapsulated in sodium alginate and whey protein isolate, with the addition of antacids CaCO 3 or Mg(OH) 2 . The obtained microgels were observed by scanning electron microscopy. Encapsulated and free probiotics were subjected to vitality assay under stressed conditions. Furthermore, dried apple snack was evaluated as a carrier for probiotics for 28 days. A significant ( p ≤ .05) effect of antacid with an encapsulating agent was observed under different stressed conditions. During exposure to simulated gastrointestinal conditions, there were observations of 1.24 log CFU and 2.17 log CFU, with corresponding 0.93 log CFU and 2.63 log CFU decrease in the case of SA + CaCO 3 and WPI + CaCO 3 respectively. Likewise, high viability was observed under thermal and refrigerated conditions for probiotics encapsulated with SA + CaCO 3 . In conclusion, the results indicated that alginate microgels with CaCO 3 are effective in prolonging the viability of probiotics under stressed conditions.
The current study was conducted to elucidate the impact of encapsulation on the stability and viability of probiotic bacteria (Bifidobacterium bifidum) in cheddar cheese and in vitro gastrointestinal conditions. Purposely, probiotics were encapsulated in two hydrogel materials (kepa carrageenan and sodium alginate) by using an internal gelation method. Cheddar cheese was supplemented with unencapsulated/free and encapsulated probiotics. The product was subjected to physicochemical (pH, titrable acidity, moisture, and protein) and microbiological analysis for a period of 35 days of storage. Furthermore, the probiotics (free and encapsulated) were subjected to simulated gastrointestinal conditions. The initial probiotic count in cheese containing encapsulated probiotic was 9.13 log CFU/g and 9.15 log CFU/g which decreased to 8.10 log CFU/g and 7.67 log CFU/g while cheese containing unencapsulated probiotic initially 9.18 log CFU/g decreased to 6.58 log CFU/g over a period of 35 days of storage. The incorporation of unencapsulated and encapsulated probiotic affected the physicochemical, microbiological, and sensory attributes of the cheese. The encapsulated probiotic bacteria exhibited better survival as compared to unencapsulated probiotic. A 2.60 CFU/g log reduction in unencapsulated cells while just 1.03 CFU/g and 1.48 CFU/g log reduction in case of sodium alginate and K‐carrageenan, respectively, was recorded. In short, encapsulation showed protection and stability to probiotic in hostile conditions.
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