Acetaminophen (APAP) overdose is a leading cause of drug-induced hepatotoxicity and acute liver failure worldwide, but its pathophysiology remains incompletely understood. Fibroblast growth factor 21 (FGF21) is a hepatocyte-secreted hormone with pleiotropic effects on glucose and lipid metabolism. This study aimed to investigate the pathophysiological role of FGF21 in APAP-induced hepatotoxicity in mice. In response to APAP overdose, both hepatic expression and circulating levels of FGF21 in mice were dramatically increased as early as 3 hours, prior to elevations of the liver injury markers alanine aminotransferase (ALT) and aspartate aminotransferase (AST). APAP overdose-induced liver damage and mortality in FGF21 knockout (KO) mice were markedly aggravated, which was accompanied by increased oxidative stress and impaired antioxidant capacities as compared to wild-type (WT) littermates. By contrast, replenishment of recombinant FGF21 largely reversed APAP-induced hepatic oxidative stress and liver injury in FGF21 KO mice. Mechanistically, FGF21 induced hepatic expression of peroxisome proliferator-activated receptor coactivator protein-1a (PGC1a), thereby increasing the nuclear abundance of nuclear factor erythroid 2-related factor 2 (Nrf2) and subsequent up-regulation of several antioxidant genes. The beneficial effects of recombinant FGF21 on up-regulation of Nrf2 and antioxidant genes and alleviation of APAP-induced oxidative stress and liver injury were largely abolished by adenovirusmediated knockdown of hepatic PGC-1a expression, whereas overexpression of PGC-1a was sufficient to counteract the increased susceptibility of FGF21 KO mice to APAP-induced hepatotoxicity. Conclusion: The marked elevation of FGF21 by APAP overdose may represent a compensatory mechanism to protect against the drug-induced hepatotoxicity, by enhancing PGC-1a/Nrf2-mediated antioxidant capacity in the liver. (HEPATOLOGY 2014;60:977-989) See Editorial on Page 792 A cetaminophen (APAP) is a commonly used over-the-counter analgesic drug with an excellent safety profile when administered in proper therapeutic doses. However, overdose of APAP can cause acute and severe liver injury. In many developed countries, APAP-induced hepatotoxicity has replaced viral hepatitis as the most common cause of acute hepatic failure and is the second most common cause of liver failure requiring transplantation.1 Mechanistically, APAP is metabolized by the cytochrome P450 system into its reactive intermediate N-acetyl-pAbbreviations: Aco, acyl-coenzyme A oxidase; ALI, acute liver injury; ALT, alanine transaminase; AST, aspartate aminotransferase; APAP, acetaminophen; CYP2E1, cytochrome P450 2E1; CYP4A10, cytochrome P450 4A10; FGF21, fibroblast growth factor 21; G6PDH, glucose-6-phosphate dehydrogenase; GCL-c, g-glutamylcysteine ligase catalytic subunit; Gpx-1, glutathione peroxidase-1; GSH, glutathione; JNK, c-Jun N-terminal kinase; NAC, N-acetylcysteine; NAPQI, N-acetyl-p-benzoquinone imine; Nox-2, NADPH oxidase-2; Nrf2, nuclear factor erythroid 2-r...