Tomato (Solanum lycopersicum L.) is economically important crop in China and elsewhere where it is grown. A previously unreported basal rot of tomato affecting as much as 15% of the stems was firstly observed in greenhouses in Northwest China (Jingyuan County, Baiyin City, Gansu Province) in March of 2015, and then the same disease of tomato was observed every year from 2016 -2021 in the region. The loss of local agricultural income caused by this plant disease in greenhouse is up to 2 million ¥ (CNY) each year. Symptoms on the basal stems were initially characterized by water-soaked lesions, which and developed rapidly into a soft, watery, decayed mass within 5 to 10 days after their appearance. Ten symptomatic stem samples were collected from basal stems exhibiting decay and were processed for microbiological analysis. Three strains of the bacteria (BBSR1~3) isolated from symptomatic samples were gram-negative, rods with a single polar flagellum, 0.5 to 1.0 × 1.5 to 3.5 µm in size. Colonies on nutrient agar (NA) medium were round, smooth, translucent, with flat edges and elevated center (“fried egg” appearance), and yellowish green to light purple in color. All isolates were positive for oxidase, gelatin liquefaction, denitrification, lipase, arginine dihydrolase, growth at 41 oC; utilization of mannitol, and production of pyocyanin. In contrast, they were negative for levan formation, lecithinase, hydrosis of starch, growth at 4 oC, utilization of maltose, xylose, γ-aminobutyrate and ethylene glycol; and growth in media supplemented with 8.5% NaCl. The representative bacterium (BBSR1) was identified as Pseudomonas aeruginosa using Biolog based Bacterial Identification System version 4.2 (Biolog Inc., Hayward, CA), revealing 0.88 similarities to P. aeruginosa in the Omnilog Biolog database. The identity was further confirmed as P. aeruginosa based on 16S rRNA sequence (1,387 bp) analysis with universal primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-GGTTACCTTGTTACGACTT-3'). A BlastN search of GenBank revealed a 100% identity to that of Pseudomonas aeruginosa strains JCM5962 (LC069033), SM-1 (KP794924), and KUN4 (KR025537). The 16S rRNA gene sequence of strain BBSR1 (1,387 bp) was deposited in GenBank (KT454095). The representative strain BBSR1 was further evaluated in pathogenicity tests. 1-month-old tomato (cv. Tomo608) potted plants were spray inoculated with BBSR1 bacterial suspensions (≈ 3×108 CFU/ml) and wounded with a sterilized needle prior to inoculation. Controls were wounded similarly and inoculated with sterile water. This experiment was maintained at 28°C with 80 to 85% relative humidity in a greenhouse. Three replicates were used for each treatment and three mature plants in each pot. Symptoms developed within 14 days and were similar to those originally observed, whereas symptoms did not occur on the control plants. Strains recovered on NA were identified as P. aeruginosa based on physiological and biochemical characteristics and Biolog (Biolog, Hayward, CA). Although Pseudomonas aeruginosa is generally regarded as a human conditional pathogen, it has been reported to cause disease on a variety of plants including ginseng (Gao et al. 2014), tinda (Mondal et al. 2012), onion (Abd-Alla and Bashandy 2012) and sweet basil (Walker et al. 2004). This is the first report of P. aeruginosa causing basal stem rot on tomato in Gansu Province, China. Under the globalization, human beings should give great importance to the bacterial pathogens that cross kingdom to cause infection and become co-pathogenic in plant and animal.
Leaf functional traits are the essential components of adaption plant strategies and have different responses to various environments, but our knowledge of how plants adapt to highly complex urban environments through coordinated changes in leaf functional traits is limited. In this study, we studied the response of landscape plants to the environments of sports field (SF), park (PAR), residential green space (RES), and greenway (GW), and analyzed the effects of the different green space environments on trade-off strategies of plants based on leaf functional traits. The results showed that leaf functional traits of plants and adaptation strategies varied among different urban environments in Lanzhou, China. Leaf length (LL), width (LW), area (LA), and special leaf area (SLA) were PAR > SF > RES > GW. Leaf nitrogen (LNC) and phosphorus content (LPC) were SF > PAR > RES > GW. Leaf carbon content (LCC), leaf dry matter content (LDMC), the ratio of leaf carbon and nitrogen (C/N), and the ratio of leaf carbon and phosphorus (C/P) was GW > RES > PAR > SF. The landscape plants in SF and PAR were more adaptive to the urban environment than those in RES and GW. Among different green space environments, landscape plants in SF and PAR tended to have an acquisitive strategy with high LL, LW, LA, SLA, LNC, and LPC. In contrast, plants in RES and GW tended to have a conservative strategy with a high level of concentration of LCC, LDMC, C/N, and C/P.
The failure cause of the fracture in wave trap hanging ring in A-phase fault tripping of a 220kV line was analyzed by using macroscopic inspection of fracture, chemical composition analysis, hardness test, metallographic structure analysis and fittings load calculation. The results show that the main causes of hanging ring fracture were unqualified material and welding quality.
Through a macro inspection, chemical composition analysis, hardness inspection, graphite carbon inspection and energy spectrum analysis, the reason for the break of the energy storage spring of the circuit breaker in a 110kV substation are analyzed. The results show that poor manufacturing technology and anti-corrosion technology of the spring are the main reason for its fracture. Corresponding control measures are put forward to avoid similar failures from happening again.
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