Incubation of Zea mays cv. Nicco seedlings with 6-methoxybenzoxazolin-2(3H)-one (MBOA) led to a minor detoxification product hitherto only found in Poaceae. This new compound was identified as 1-(2-hydroxy-4-methoxyphenylamino)-1-deoxy-beta-glucoside 1,2-carbamate (1) (methoxy glucoside carbamate) and represents an analogue to the previously described 1-(2-hydroxyphenylamino)-1-deoxy-beta-glucoside 1,2-carbamate (glucoside carbamate) from benzoxazolin-2(3H)-one (BOA). In Portulaca oleracea var. sativa cv. Gelber treatment with BOA resulted in further unknown detoxification products, which were not synthesized in detectable amounts after BOA absorption in all other species tested. Compound 1 easily undergoes decay into BOA-5-O-glucoside (2). Z. mays seedlings, known to produce BOA-6-O-Glc on incubation with BOA, are able to transform BOA-5-OH into BOA-5-O-glucoside (2). Besides the known compounds, maize contained a formerly unseen product that accumulated during late stages of the detoxification process. It was isolated and identified as 1-(2-hydroxyphenylamino)-6-O-malonyl-1-deoxy-beta-glucoside 1,2-carbamate (3) (malonyl glucoside carbamate).
Dehydration of a 2-(13)C-labeled synthetic sample of the natural aglucone 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one ([2-(13)C]-DIMBOA, 10) using N-ethoxycarbonyltrichloroacetaldimine led to 3-formyl-6-methoxybenzoxazolin-2(3H)-one ((13)C-labeled FMBOA, 11) with complete transfer of the (13)C label into the -CHO group. On the basis of this finding, a mechanism for the dehydration is proposed.
Basis on the analysis of the plate flatness detection, some problems of the traditional flatness measuring method is pointed out and the 3D reconstruction method of flatness is proposed. The digital flatness measuring instrument is designed, and the measured plane information is entered into the computer, then the data processing is completed by software, which solves the problem of amounts of data, complicated calculation and low accuracy of traditional measurement method. The 3D reconstruction of surface morphology is completed by OpenGL and BCB, to make the information of flatness more sufficient and intuitive. To verify the validity of the measurement, use the flatness function module of dual-frequency laser interferometer to evaluate the instrument accuracy, the results show that it reaches the expected precision index.
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