The transplantation of embryonic stem cells can effectively improve the creeping strength of nerves near an injury site in animals. Amniotic epithelial cells have similar biological properties as embryonic stem cells; therefore, we hypothesized that transplantation of amniotic epithelial cells can repair peripheral nerve injury and recover the creeping strength of the brachial plexus nerve. In the present study, a brachial plexus injury model was established in rabbits using the C6 root avulsion method. A suspension of human amniotic epithelial cells was repeatedly injected over an area 4.0 mm lateral to the cephal and caudal ends of the C6 brachial plexus injury site (1 × 106 cells/mL, 3 μL/injection, 25 injections) immediately after the injury. The results showed that the decrease in stress and increase in strain at 7,200 seconds in the injured rabbit C6 brachial plexus nerve were mitigated by the cell transplantation, restoring the viscoelastic stress relaxation and creep properties of the brachial plexus nerve. The forepaw functions were also significantly improved at 26 weeks after injury. These data indicate that transplantation of human amniotic epithelial cells can effectively restore the mechanical properties of the brachial plexus nerve after injury in rabbits and that viscoelasticity may be an important index for the evaluation of brachial plexus injury in animals.
Selecting accurate hydraulic conductivity is critical to calculating correctly seepage field. Because of geotechnical hydraulic conductivity with spatial randomness and its errors in measurement and computation, the extended Kalman filter (EKF) is proposed to its back analysis. In conjunction with the finite element method (FEM) of seepage, details of the EKF are introduced in this paper. With FORTRAN programs, the hydraulic conductivity is revised according to the real-time monitoring data during tunnel excavation, and illustrates the EKF merits of considerable convergence and tolerable accuracy.
Based on the numerical simulation method and actual measurement analyses, characteristics of working face mineral pressure of the River Mouth Mine has been analyzed and this paper has obtained the following results: First weighting step of the main roof is 23.2 m, the average dynamic pressure coefficient is 1.54~1.74, mine strata behaviors is fiercer; hydraulic prop’s average working resistance is 45.72 KN of each, which indicates hydraulic prop’s working resistance has larger surplus coefficient, some measures should be taken to increase utilization ratio; advanced support length of return airway and mechanical roadway should reach to 30 m, the method and results can be used to improve support pattern of working face and guide safety production.
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