No abstract

By using an expressed sequence tag bioinformatic algorithm, we identified that Lin28 homolog B (Lin28B) may have an oncofetal expression pattern which may facilitate detecting cancer cells in adults. It is also reported to be a potential marker for cancer stem cells. Therefore, we sought to verify oncofetal-stemness characters of Lin28B and test its potential as a circulating cancer stem cell-like marker in adult HCC patients. Lin28B mRNA was examined in a panel of fetal tissue, adult tissue and tumors. Lin28B was over-expressed or knocked down in HepG2 cells to evaluate its potential as a stem cell-like marker. RT-qPCR for Lin28B was performed in the peripheral blood mononuclear cells from patients with HCC receiving surgery (n=96) and non-HCC controls (n=60) and analyzed its clinical significance. Lin28B showed an oncofetal expression pattern. Its overexpression could upregulate stemness markers (OCT4, Nanog and SOX2) and enhance tumorsphere formation in vitro. Lin28B knockdown had opposite effects. Circulating Lin28B was detected in peripheral blood mononuclear cells in 3 cases (5%) of non-HCC controls and 32 cases (33.3%) of HCC patients. In HCC patients, circulating Lin28B was associated with high tumor grade (P=0.046), large size (P=0.005), high AJCC stage (P=0.044) and BCLC stage (P=0.017). Circulating Lin28B was significantly associated with decreased recurrence-free survival (P<0.001). Circulating Lin28B separated early stage HCC into 2 recurrence-free survival curves (P=0.003). In multivariate analysis, circulating Lin28B was an independent variable associated with early recurrence (P=0.045) and recurrence in early stage HCC (P=0.006). In conclusion, the oncofetal gene Lin28B is a potential oncofetal cancer-stem-cell-like circulating tumor cell marker that correlates with HCC recurrence after hepatectomy. Circulating Lin28B could refine early AJCC stages. Our finding supports the possible use of a TNMC (C for circulating tumor cells) staging system in HCC.

Introduction: Exercise intervention is the easiest and most effective way to promote human health. This study combined technology and exercise to improve the health behavior of the older adults through a physical activity intervention and to enhance a health-promoting lifestyle. Materials and methods: A quasi-experimental research method was used to openly recruit 120 healthy male and female older adults over 65 years old (average age of males: 71.6 ± 1.25 years; average age of females: 72.3 ± 1.28 years), all of whom wore smart bracelets. The participants were monitored by special personnel during the same period of walking every Monday to Friday. All participants recorded their daily steps, distance walked, and calorie consumption data for a period of 8 weeks. Results: After 8 weeks of walking, all participants showed a positive medium–high correlation of various factors between healthy behaviors and the health-promoting lifestyle scales. In the post-tests of each factor of two scales, males had the highest correlation between regular physical activity and physical activity, and females had the highest correlation between regular physical activity and social support. The variabilities in the explanatory power of the health behaviors of males and females on the health-promoting lifestyle were R2 = 70.9% (p < 0.01) and R2 = 74.1% (p < 0.01), indicating that the variables of healthy behaviors have a positive effect on health-promoting lifestyles in male and female older adults. Conclusions: Walking interventions positively affect the health behaviors of older adults and encourage health-promoting lifestyles. The value of this study is in its contribution to health promotion and public health recommendations for older adults.

The purpose of this study was to understand the effect of intervention in the form of physical activity and social support while co-exercising to promote a health-promoting lifestyle in older adults. This study openly recruited 60 healthy male older adults, all of whom wore a wrist-worn Garmin device walker. Experimental group A was monitored by a special person and walked together as a group, experimental group B walked independently outdoors, and the control group went about their daily routine as normal. All participants recorded daily steps and calorie consumption data for eight weeks. The results of the study showed that when walking together in experimental group A, the number of daily walks and calories consumed could be maintained at a high level due to the effect of group and social support. The number of daily walks in experimental group B was inconsistent due to the lack of motivation to walk on their own. The control group consumed the lowest number of calories in their daily routine. The results show that physical activity and social support have positive explanatory power for a health-promoting lifestyle. Finally, physical activity in older adults should be promoted, and social support for interaction with peers can effectively promote a healthy lifestyle and respond to the arrival of the super-aged society in advance.

The purpose of this study was to understand the effects of a physical activity program and high-protein supplementation on body composition and upper and lower extremity muscle strength in male older adults in rural areas. In this study, 60 healthy male older adults (mean age 77.5 ± 4.6 years) from rural areas were recruited and randomly assigned to experimental group A (intervention of the physical activity program and high-protein supplementation), experimental group B (daily routine, with only intervention of high-protein supplementation), or control group C (daily routine). Experimental group A (EGa) carried out a physical activity plan three times a week, with an exercise intensity and calorie consumption of 250 kcal (5METs × ⅔hr × 75) for 3 months and drank a high-protein supplement (1.3 g/kg BW/day) after each exercise; experimental group B (EGb) followed only the intervention of high-protein supplementation. All the participants underwent pre- and post-tests for body composition, waist–hip circumference (WC, HC), handgrip strength (HS), 30 s dominant arm curl, 30 s sit to stand, and 2 min step tests. The results of the study showed that EGa significantly decreased body mass index (BMI), body fat mass (BFM), body fat percentage (BFP), WC, HC, and waist-to-hip ratio (WHR) and increased basal metabolic rate and muscle mass. Although both EGa and EGb used high-protein supplementation, EGa’s added three-month intervention of a physical activity program made it easier for that group to increase muscle mass and muscle strength. The WHR decreased from 1.015 to 0.931, representing a decrease of 8.28%, and an obvious weight loss effect was achieved. Thus, we concluded that the best way to maintain muscle strength in older adults is through physical activity with resistance and protein supplementation, which can reduce muscle loss in older adults.

The purpose of this study was to evaluate the speed, agility, and explosive strength performance of elite basketball players over an 8-week plyometric training program. Fifteen elite male college basketball players in Taiwan (average age 22.16 ± 0.85 years old) were publicly recruited. All participants received 24 plyometric training courses three times per week for 8 weeks, and the courses were implemented pre- and post-test. The speed and agility test items were divided into a 20 m sprint and a T-shaped run. In the explosive strength test, a force plate was used to measure countermovement jump to understand the pre- and post-test differences in all the test indicators, including the rate of force development, time of the rate of force development, ground reaction forces for the moment of jumping, duration of passage, and jump height. It was found that, after the participants underwent the plyometric training program, the body mass index and body fat percentage were significantly reduced, the skeletal muscle mass was significantly increased, and the post-test scores for speed and agility improved significantly. All the participants exhibited a steeper gradient for the rate of force development (r = −0.816~−0.963) and a shorter time for the rate of force development (0.107~0.232 s). The ground reaction forces reached 1509.61~2387.11 Newtons. The duration of passage reached 0.643 s, and the jump height reached 0.624 m. The conclusion was that the plyometric training program can increase muscle volume in the lower limbs and legs, increase the rate of force development, and shorten the jumping time, thereby enhancing explosive strength.