Aim: to compare the push out bond strength of the root canal filling with epoxy resin-based (Adseal), MTA-based (MTA Fillapex) and Bioceramic-based (Endosequance BC) sealers. Materials and Methods:Forty five extracted mandibular human first premolars with straight fully formed roots were selected for this study and were de-coronated at 17 mm from the apex. All root canals were prepared by using the ProTaper Universal Ni Ti rotary system in a crown down manner up to F4 according to manufacturer's instruction to the their working length. The canals were irrigated with 17% EDTA to remove the smear layer and the final rinse was performed with 5 mL 5.25% sodium hypochlorite. The roots were randomly assigned to three groups, fifteen for each (n=15). Adseal, MTA Fillapex and Endosequence BC sealers were used with gutta percha size F4 by lateral condensation technique for groups I, II and III respectively. All roots stored in 100% humidity at 37ºC for one week for completing setting of sealers. The roots were embedded in self cure acrylic resin, and transversely sectioned perpendicular to the long axis of the root 2 mm thickness slices. Apical diameter of 0.88 ± 0.02 mm in all slices were selected for the pushout test by universal testing machine. The dislodging force was measured in Newton for all slice samples and tabulated. Push out bond strength (MPa) for every slice sample is calculated and the three groups were compared. The slices were then examined under stereomicroscope and categorized into adhesive, cohesive or mixed type of bond failure mode. The data were tabulated and statistically analyzed.Results: Endosequence bioceramic represented the highest bond strength followed by Adseal then MTA Fillapex which represented the lowest bond strength with a significant difference between them at P≤0.05. Comparison between Adseal and Endosequence bioceramic groups and between MTA Fillapex and Endosequence bioceramic groups showed a significant difference between them at P≤0.05 with higher bond strength of Endosequence bioceramic sealer. Adseal represented higher bond strength than MTA Fillapex with no significant difference between them at P≤0.05. Comparison between the three tested groups of sealers regarding the bond failure categories showed no significant difference between them at P≤0.05. (1708) Recently, MTA Fillapex (Angelus, Londrina, PR, Brazil) is MTA-based sealer presented in a paste-paste system and composed of resins, bismuth oxide, silica nanoparticles, and pigments. It is a modification of the MTA original formulations to improve the characteristics such as good radiopacity, flow, setting time, alkaline pH and adhesion, Conclusion: Within the limitations of this study, Endosequence bioceramic sealer show promising results as root canal sealer and exhibited a greater resistance to push out than MTA Fillapex and epoxy resin-based sealer (Adseal) with gutta-percha as a root canal filling.
INTRODUCTION: Remaining microorganisms after chemo-mechanical cleaning and disinfection may be eliminated or rendered harmless by entombing them through complete obturation with gutta percha points and sealer. Adequate antimicrobial effect is of optimum importance to achieve root canal treatment success. OBJECTIVES: The aim of this study was to investigate the antimicrobial potential of two types of sealers against Enterococcusfaecalis present in dentinal tubules, over a 30 day period. MATERIALS AND METHODS: Cells of Enterococcus faecalis were introduced into the dentinal tubules by incubation in brain-heart infusion broth for 3 weeks. AH Plus and Endosequence BioCeramic sealer (BC sealer) were coated on the root canal wall of the dentin specimens and cold lateral obturation technique was preformed, specimens were then stored for one, seven, and 30 days at 37°C. At the end of each incubation period, dentin powder was collected by sequential removal of dentin by the use of peeso reamers throughout the sample thickness, total bacterial count was assessed by Colony forming units (CFU) count. RESULTS: No significant difference was observed in the total bacterial count among the three groups in day one. AH Plus showed statistically significant lower bacterial counts in comparison to the positive control group in day 7 and day 30, while Endosequence BC showed a statistically significant lower bacterial count in comparison to the positive control group at day 30. CONCLUSIONS: AH plus exhibited a stronger antibacterial affect after setting and therefore is more effective than Endosequence BC in eliminating Enterococcus faecalis from the root canal system. In addition, both sealers lose their antibacterial effect with time.
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