In this study, we report the female-lineage mitochondrial genome of Xenostrobus atratus for the first time. The circular mitochondrial genome is 14,806 bp in length and contains 12 protein-coding genes, 22 transfer RNA genes, and two ribosomal RNA genes. All genes are encoded on the heavy strand. The genome composition is A + T biased (66.6%), with 25.2% A, 41.4% T, 21.7% G and 11.7% C. A Bayesian inference (BI) phylogenetic tree was constructed based on the mitochondrial genomes of X. atratus and 46 other Mytilidae species. Our results demonstrate that X. atratus and Limnoperna fortune i have distinct lineages, opposing synonymizing Xenostrobus within Limnoperna . According to this study, the validity of the subfamily Limnoperninae and genus Xenostrobus is strongly supported. However, there is still an urgent need for more mitochondrial data to decide to which subfamily X. atratus belongs.
Backgrounds Mussels have been one of the most abundant and highly evolved marine biological groups with high level of genetic differentiation between and within species. The yellow-banded horse mussel, Modiolus modulaides (Röding, 1798), is an important economic species in Philippines and an endangered species in Japan, with no whole mitogenome characterized. Methods and Results In this study, we sequenced the whole mitochondrial genome of Modiolus modulaides (Röding, 1798), a medium-sized mussel collected from Guangxi, China. Result shows a 15,422 bp closed-circular DNA molecule, including 12 protein-coding genes, 22 transfer RNA genes and two ribosomal RNA genes. The genome composition is obviously A+T biased (62.5%), with 23.1% A, 39.4% T, 25.4% G and 12.0% C, making AT-skew and GC-skew being -0.26 and 0.36, respectively. Phylogenetic analysis of Modiolus modulaides and other 36 mussel species based on 12 protein-coding genes and two ribosomal RNAs of mitogenomes shows that Modiolus modulaides is most closely related with Modiolus phillippinum (Hanley, 1843).Conclusions A hypothesis that the current genus Modiolus should be separated three distinctive genera was raised based on morphological and phylogenetic characteristics, as well as gene patterns of mitogenomes. These results put forward new insight of phylogenetic relationship of Modiolus species and will help to further study the taxonomy and phylogeny of Mytilidae.
Background Andrographolide is a diterpenoid derived from Andrographis paniculate, which has anti-inflammatory, antibacterial, antiviral and hepatoprotective activities. Gram-negative bacterial infections can cause varying degrees of liver injury in chickens, although andrographolide has been shown to have a protective effect on the liver, its underlying mechanism of action and effects on liver proteins are not known. Methods The toxicity of andrographolide on the viability of LMH cells at different concentrations and times was analyzed by CCK-8 assays. Alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in the culture supernatants were measured using an automatic biochemical analyzer to evaluate the protective effect of androscopolide on LPS-induced injury of LMH cells. Subsequently, TMT proteomics analysis were performed on the NC, LPS, and LPS-Andro groups, and bioinformatics analysis was performed on the differentially expressed proteins (DEPs). Results It was found that andrographolide reduced ALT and AST levels in the cell supernatant and alleviated LPS-induced injury in LMH cells. Proteomic analysis identified 50 and 166 differentially expressed proteins in the LPS vs NC group and LPS-Andro vs LPS group, respectively. Andrographolide may be involved in steroid metabolic processes, negative regulation of MAPK cascade, oxidative stress, and other processes to protect against LPS-induced liver injury. Conclusions Andrographolide protects against LPS-induced liver injury, HMGCS1, HMGCR, FDPS, PBK, CAV1, PRDX1, PRDX4, and PRDX6, which were identified by differential proteomics, may be the targets of andrographolide. Our study may provide new theoretical support for andrographolide protection against liver injury.
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