Soil contamination by toxic trace metal elements, like barium (Ba), may stimulate various undesirable changes in the metabolic activity of plants. The plant responses are fast and with, direct or indirect, generation of reactive oxygen species (ROS). To cope with the stress imposed by the ROS production, plants developed a dual cellular system composed of enzymatic and non-enzymatic players that convert ROS, and their by-products, into stable nontoxic molecules. To assess the Ba stress response of two Brassicaceae species (Brassica juncea, a glycophyte, and Cakile maritime, a halophyte), plants were exposure to different Ba concentrations (0, 100, 200, 300 and 500 µM). The plants response was evaluated through their morphology and development, the determination of plant leaves antioxidant enzymatic activities and by the production of plants secondary metabolites. Results indicated that the two Brassicaceae species have the ability to survive in an environment containing Ba (even at 500 µM). The biomass production of C. maritima was slightly affected whereas an increase in biomass B. juncea was noticed. The stress imposed by Ba activated the antioxidant defense system in the two species, noticed by the changes in the leaves activity of catalase (CAT), ascorbate peroxidase (APX) and guaicol peroxidase (GPX), and of the secondary metabolites, through the production of total phenols and flavonoids. The enzymatic response was not similar within the two plant species: CAT and APX seem to have a more important role against the oxidative stress in C. maritima while in B. juncea is GPX. Overall, total phenols and flavonoids production was more significant in the plants aerial part than in the roots, of the both species. Although the two Brassicaceae species response was different, in both plants catalytic and non-catalytic transformation of ROS occurs, and both were able to overcome the Ba toxicity and prevent the cell damage.

Soils and water resources of our ecosystems may contain Barium (Ba), a toxic metal naturally existent in the Earth’s crust and also can be derived from recycled wastes produced of several anthropogenic activities. As a result of this fact, the accumulation of Ba in agriculture soils would increase to reach the crops and eventually end up in the human food chain. The purpose of this work was to study tolerance and accumulation abilities in Limbarda crithmoides and Helianthus annuus treated with increasing concentrations of barium (from 0 to 500 µM) for 45 days. In order to evaluate the response of these species to Ba stress, the biomass production, the water status, and the accumulation of the secondary metabolites, macronutrients, total inorganic nitrogen (TIN), and Ba in shoots and roots, as well as chlorophyll levels, and metal tolerance index of the entire plant were assessed. Results showed an increase in plant biomass production and tolerance index in the two species with increasing Ba concentration. A significant increase in polyphenols and flavonoids levels was also shown with no negative effect on the macronutrients and TIN; however, the latter were found reduced in roots of L. crithmoides. Chlorophylls also were not affected. An average of 3000 µg·g−1 DW of Ba was accumulated in each organ of L. crithmoides while H. annuus accumulated up to 1350 µg·g−1 DW in the shoots. Our findings proved that L. crithmoides and H. annuus were susceptible to tolerate Ba-induced stress with high levels of Ba accumulation in the aboveground parts as well as in the roots during the 45 days of the experiments.

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Rare earth elements (REEs) present a group of nonessential metals for the growth and development of plants. At high concentrations, they can induce internal stress and disturb the physiological and biochemical mechanisms in plants. The potential uptake of lanthanum (La) and cerium (Ce) by the horticultural plant Helianthus annuus and the effect of these elements on its growth, its absorption of macroelements, and the contents of phenolic compounds and flavonoids were assessed. The plants were exposed to 0, 1, 2.5, 5, and 10 µM of La and Ce for 14 days. The results showed a remarkable accumulation of the two REEs, especially in the roots, which was found to be positively correlated with the total phenolic compound and flavonoid content in the plant shoots and roots. The plant’s growth parameter patterns (such as dry weight and water content); the levels of potassium, calcium, and magnesium; and the tolerance index varied with the concentrations of the two studied elements. According to the tolerance index values, H. annuus had more affinity to La than to Ce. Although these metals were accumulated in H. annuus tissues, this Asteraceae plant cannot be considered as a hyperaccumulator species of these two REEs, since the obtained REE content in the plant’s upper parts was less than 1000 mg·Kg−1 DW.

The continuous progress of global manufacturing and anthropogenic activities has resulted in excessive environmental metallic pollution, particularly with rare earth elements (REEs) which have become a prevalent issue of global concern due to their high toxicity and widespread existence. REEs-contaminated soils could ruin agriculture by inducing plant physiology disturbances in various crops that are considered the principal link of the human food chain. The main purpose of the present work is to assess the phytotoxicity of two light REEs, lanthanum (La) and cerium (Ce), in Helianthus annuus after 14 days of exposure to different concentrations of La and Ce (0, 1, 2.5, 5, and 10 µM). Plants showed different variations in shoot and root lengths at the end of the trial period. The accumulation of photosynthetic pigments, such as chlorophylls and carotenoids, as well as the photosynthetic efficiency, the non-photochemical quenching, the photosynthetically active radiation, and the electron transport rate, increased in the two REE treatments. Hydrogen peroxide significantly increased in all applied concentrations of La and Ce. A significant increase in malondialdehyde content was noticed only when plants were exposed to 2.5 µM La and 10 µM Ce. Results also demonstrated that La and Ce induced an increase in the activity of superoxide dismutase, peroxidase, and catalase (only the highest concentration of La decreased catalase activity). The exposure to different REE concentrations induced the accumulation of La and Ce in the plants, mainly in roots. Helianthus annuus showed an effective resistance behaviour facing La- and Ce-induced stresses.

In the present work, the effectiveness of chlorophyll a fluorescence as a quick tool to detect the effect of Pb–stress on Limbarda crithmoides and Helianthus annuus species, when exposed to increasing Pb concentrations (0–500 µmol.L–1) for 45 days, was evaluated. The chlorophyll level in plant leaves, as well as the fresh weight and Pb content in the shoots and roots in both plant species, were also determined. L. crithmoides did not show any significant variation in photochemical quenching parameters and energy fluxes in all Pb concentrations despite the change in OJIP plot of 100 µmol.L–1 Pb. In addition, a significant increase in chlorophyll a content was noted along with an increase in the biomass production. However, in H. annuus, Pb stress increased energy absorption, dissipation and trapping and decreased energy transport and electron transport rate. In addition, the OJIP curve showed a notable modification at 300 and 500 µmol.L–1 Pb, without significant changes in the chlorophyll contents and in the biomass production under all Pb concentrations. Results also revealed that Pb was accumulated in the shoots and roots of L. crithmoides and H. annuus and the accumulation was more notorious in the roots of the treated plants. Lead exhibited different influence on the photosystem II performance in both species, thus, the evaluation of chlorophyll fluorescence was a very efficacious tool to reflect the physiological status of L. crithmoides and H. annuus under Pb stress.

The aim of this work is to evaluate the degree of degradation phenolic compounds in olive mill wastewater (OMW), using crude plant peroxidases. In fact, OMW was treated with crude peroxidases extracted from radish (Raphanus sativus L.) and nettle (Urtica urens L.) leaves. A significant reduction of more than 60% of total phenols was observed in OMW incubated with peroxidase crude extract during seven days. The present chromatographic data obtained by high performance liquid chromatography (HPLC) show that enzymatic treatment may change the composition of the polyphenols contained in the OMW. Thus, we observed a significant decrease in some phenolic acid levels, such as gallic acid, p-coumaric acid and hydroxytyrosol, and the disappearance of vanillic acid, compared to the non-treated OMW. Finally, phytotoxicity of the treated and non-treated OMW was tested by means of young sunflower plants (Helianthus annuus) grown in hydroponic medium. Our results showed that sunflower plants grow normally when the nutrient medium contains treated OMW; whereas they fade rapidly in the presence of non-treated OMW. We conclude that treatment of OMW with radish and nettle crude extracts could attenuate OMW phytotoxicity considerably.