A field experiment was carried out at the Horticulture Farm of the Bangladesh Agricultural University, Mymensingh to evaluate the effects of micronutrients and their methods of application on growth and yield of potato cv. Diamant during the period from November 2019 to March 2020. The experiment consisted of two factors, Factor A: Two methods of micronutrient application viz., M1= Basal and M2= Foliar, and Factor B: Four levels of micronutrients viz., T0= control treatment, T1= Boron (B) @ 2 kg/ha, T2= Zinc (Zn) @ 3 kg/ha, T3= B @ 2 kg/ha + Zn @ 3 kg/ha. The experiment was laid out in split plot design with three replications. Result of the experiment revealed that application method and different levels of micronutrients alone or in combination significantly influenced all the parameters studied. The highest tuber yield (3.53 t/ha) was obtained with foliar application and the lowest was found from basal application. On the other hand the highest tuber yield (4.56 t/ha) was obtained when B + Zn @ 2 kg B/ha + 3 kg Zn/ha was applied and the lowest was recorded from control treatment. Among the treatment combinations, foliar application of micronutrients along with combined treatment of B + Zn @ 2 kg/ha + 3 kg/ha produced the maximum tuber yield (4.89 t/ha) while the lowest was obtained from the control treatment. Therefore, foliar application method along with the combined treatment of boron plus zinc was found to be better in respect of growth and yield of potato.
Toxic metal mercury (Hg) in poultry feed might affect seriously on the environment and human being. So continual or gradual analyses of toxic metal contents in poultry feedstuff are of prime significance for assessing the feed quality and consumer safety. In this study, a method named Mercury Vapor Unit Atomic Absorption Spectrometry (MVU-AAS) was used to analyze the content of Hg in poultry feed in compliance with the Council Directive 333/2007/EC, Commission Decision 657/2002/EC. The metal Hg was analyzed from the feed sample inthe laboratory by MVU-AAS (Model: AA-7000 Shimadzu, Japan) and the absorbance was read at 253.65 nm wavelength, The method was confirmed and validated by complying with the international guidelines and assessing a number of criteria say system suitability, precision studies, linearity check, uncertainty measurement and recovery % etc.. The results revealed that the coefficient of variation (CV%) for system suitability and precision was <10% for the metal (Hg) detected in this study. The linearity of the calibration curves was excellent (r2>0.998) at various concentrated levels. The limits of detection (LoD) value in feed was 0.007 mg/Kg and the limit of quantification (LoQ) value in feed sample was found 0.023 mg/Kg. The values of instrumental detection limit (IDL) and instrument quantification limit (IQL) were 0.045(μg/l), and 0.15(μg/l), respectively. The recovery (%) found for the metal was 92.29 %. The overall CV% of repeatability and reproducibility ranged from 3.27 % to 5.92 %. The value ranges from 12 to 16 was observed for the measurement uncertainty (%) of Hg in this study. The proposed validation criteria indicate that it is a reliable method that can be used easily for the routine analysis of toxic metal (Hg) content in poultry feed sample.
The experiment was performed by applying different concentration levels of ascorbic acid and yucca extract to assess the production performance of broiler chickens during heat stress. A trial of 300 day-old commercial broiler chicks was carried out on littered floor for a period of 4 weeks. The chicks were randomly allocated into five treatments and each dietary treatment consisted of 4 replicates having 15 chicks in each of the replication. The experimental treatments were follows:T1(control group without any additives),T2(1ml YE with 16L drinking water), T3(200 mg AA per kg feed), T4(1ml YE with 16L drinking water and 200 mg AA per kg feed) and T5(1ml YE with 20L drinking water and 150 mg AA per kg feed). The findings revealed that there had significant effects (P<0.05) in body weight gain, feed consumption and FCR value. Among the treatment groups FCR value found better in T4 group. The results also revealed that the treatments had significant effects(P<0.05) in dressed wings, breast, back, thigh, drumstick. However, in treatment T4 group the carcass weight is better than the other treatment groups.The Hemoglobin, WBC, RBC, Neutrophil, Lymphocyte, Monocyte, PCV and MCV was statistically insignificant(P>0.05) among different treatments.In economic aspect, though total expenditure was lower in control group but higher profit per bird was found in other treatment groups. Therefore, this study suggested that the Yucca extract and ascorbic acid perform better in broiler rearing even they are on stressed condition.
The study was undertaken to assess the quality of broiler meat via detection of heavy metal contents. Seven farms were selected randomly from the five divisions of Bangladesh. Broiler meat samples were collected during starting and growing phases of production stage from each of the farms by purchasing to detect the heavy metal contents i.e chromium (Cr) and cadmium (Cd) in this study. A total of 210 meat samples (105 starter and 105 grower) was collected from the selected farms and analyzed to determine the concentration of toxic metals (Cr, Cd) in meat samples. The heavy metal data (Cr, Cd) of broiler starter meat samples from laboratory analyses of five divisions of seven feed mills were unaffected (P>0.05) between treatment. The Cd contents of broiler grower meat samples of five divisions of seven farms together differed significantly (P<0.05) between treatment except for Cr. Significant variation (P<0.01) was observed in the various meat samples of broiler chicken by individual division (Dhaka, Chittagomg). It can be concluded that the quality of broiler meat of different farms appears to be good based on the chemical evaluation, even though variation was found in the toxic metal contents of meat samples. The analyzed concentration of toxic metals in meat samples were within the range or limits of the acceptable level, so no possibility of causing health hazard will create over the consumer world through after consumption of broiler meat.
Milk is considered as an ideal food item, and it can be contaminated with the toxic metal (Pb) by various ways. The metal lead (Pb) exposes toxicity greatly when it accumulates gradually inside the body cavity of human or animal. The study is aimed at the detection of poisonous mineral lead in cow’s milk sample by Graphite Furnace Atomic Absorption Spectrometry (GF-AAS) method.Besides, public health concern or issues in the food chain of the consumer world is also taken into consideration in this study. Though the method is a bit troublesome, but an attempt was made herein this study to make it easy access for the determination and method validation for the analysis of mineral content in milk sample by complying with the Council Directive 333/2007/E. For the ratification of this method, a pretty good number of criteria including linear range, limits of detection and quantifications, accuracy (%), measuring uncertainty, repeatability and reproducibility or precision checks etc., were assessed for the affirmation of the method. GF-AAS (Model: AA-7000 Shimadzu, Japan) technique was used for the analysis of poisonous element lead in dairy cow milk samples. Lastly, detection of Pb in milk samples was done with ultraviolet/visible detection set at 283.0 nm wave length. The method was confirmed complying with the international guidelines and acceptance values for system suitability, precision, linearity, uncertainty and accuracy or recovery % were met in all aspects. The Relative Standard Deviation (RSD) or Coefficient of Variation (CV%) for system suitability and precision was <10% for the metal (Pb) measured in the milk sample. The linearity of the calibration curves was excellent (r2>0.999) at various concentrations for the lead. The instrumental Limits of Detection (LoD) value in milk were 0.397 and the limits of quantification (LoQ) value in milk samples was 1.32μg//L, respectively, for Pb, And the method of LoD and LoQ for Pb being 0.993 and 3.30 μg/Kg, respectively. The overall recovery (%) found 98.98 for the metal (Pb). The overall RSD or CV% of reproducibility and repeatability percentages being 6.85 and 7.65%, respectively. The value for measurement uncertainty (%) was 7.0 for Pb. The developed validated parameters denote that it is an easy and economical method that can be applied greatly for the regular laboratory analysis of trace mineral element in cow milk samples. After all, the potential health of the consumer health or public health concern should not be ignored at all for the continual ingestion of toxic element contained in the milk, even though the toxic mineral content found in the supplied milk samples were within the acceptable range.
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