The objectives of this work were to describe some morphometric characteristics and to establish quantitative parameters of different regions of the equine oviductal mucosa from the isthmus, ampullaryisthmic junction (AIJ), and ampulla. Twenty-one mixed-bred mares were used for this study. Mares were selected in the following reproductive phases: anestrus, estrus, and diestrus. The left oviducts were examined with light microscopy, and rights ones were studied through the intraoviductal molds. The isthmus showed the smallest luminal area, mucosal area, epithelial perimeter, and luminal diameter. On the molds surfaces, some grooves extended as longitudinal canals, reducing their depth as they approached to the AIJ. Several small height projections, some obliquely positioned towards utero-tubal junction, were observed in all reproductive phases. These formations may represent pockets or cul-de-sacs in the basal areas of the epithelial folds. The AIJ mucosa gradually changed from the smooth isthmic region toward highly folded ampulla. The number and complexity of epithelial folds showed moderate increase in the same way that many of the morphometric parameters. Multiple curves were observed on the molds of the AIJ, creating a zigzag path in the oviductal lumen. In the ampulla, the high branched epithelial folds occupied most of the lumen, leaving a small luminal area free. A linear relationship between epithelial perimeter and mucosal area was found.
The oviduct plays a key role in the reproductive process in mammals allowing transport, reservoir, and capacitation of sperm (Hunter RH 2008 Mol. Reprod. Dev. 75, 167-174), fertilization, and early embryo development among other events. The oviductal mucosa is organized in a vast net of folds that projects towards the lumen (Trautman A and Fiebiger J 1952 Comstock. Publ. Assoc., Ithaca, NY, USA). In a preliminary study, the presence of some glandular structures in the oviductal mucosa was noticed. Detailed histological studies have incredibly not been described in the mare. The objective of this work was to study the presence of glands in the mare’s oviduct. Mares, in good body condition, 3 to 14 years old, were selected at a local slaughterhouse. Reproductive status was determined by transrectal palpation and ultrasonography. Mares were selected in anovulatory phase (n = 8), in estrus (n = 7), at Day 1 to 2 post-ovulation (n = 6) and in diestrus at Day 7 to 8 post-ovulation (n = 7). Reproductive tracts were harvested immediately following slaughter and were placed on ice. Oviductal samples of 1.5 cm were taken from the ampulla, the ampullary-isthmic junction (AIJ), and the isthmus and placed in formalin for fixation and subsequent process for hematoxylin-eosin stain. The number of glands was counted at ×400 in 5 optical fields and was compared by non-parametric Kruskal-Wallis test. Glandular structures were observed in 100% of the oviducts. These glands were alveolar type and resembled those in the endometrium. The glands showed 2 locations: in the periphery of the mucosa (peripheral glands) and within the thickness of the mucosa folds. The peripheral glands were more abundant than those within the mucosa folds (P < 0.001). The amount of glands decreased progressively from the ampulla (5.7/5 fields) to the AIJ (4.5) to reach a minimum number in the isthmus (0.2; P < 0.05). For each oviductal region, the amount of glands did not change through the different reproductive stages (P > 0.05). The epithelium of these glands was formed by ciliated and non-ciliated epithelial cells arranged in a similar way of the epithelial surface. However, the peripheral glands were stained with lighter intensity than the epithelial cells in the mucosal surface. In all literature searched, which included several textbooks of veterinary histology, no reference to glands in the oviducts of several domestic species including the mare was found. In contrast, glands in the oviduct have been described in birds (Richardson KC 1935 Biol. Sci. 225, 149-195) where they produce albumen and the eggshell membranes, and interestingly, one article about the oviduct of the bitch (Steinhauer N et al. 2004 Reprod. Domest. Anim. 39, 110-119) showed evidence of glands in this organ. In the mare, these oviductal glands probably add a distinct secretion of the oviductal surface to the tubal fluid since they show different chromatic affinity. Further investigation is needed to understand the function of these glands in the mare oviductal physiology.
RESUMEN:La Plastinación es la técnica más moderna para la conservación de piezas anatómicas y especímenes. Esta técnica consta de cuatro etapas: fijación, deshidratación, impregnación forzada y curado. Este trabajo pretende mejorar la eficiencia en la producción de órganos plastinados mediante la técnica de Plastinación estándar denominada S 10. Se utilizaron riñones de equinos mestizos criollos provenientes del Frigorífico Aimar S/A, ubicado en la zona rural de Río Cuarto. Luego de realizar la fijación del órgano con formalina se procedió a deshidratarlo. La deshidratación se llevó a cabo en tres sub-etapas donde en cada una de ellas se trabajó a la misma temperatura y se utilizó como solvente una solución de acetona en diferentes concentraciones. Las mediciones se realizaron con acetómetro registrándose el tiempo insumido para lograr la deshidratación. Los datos se analizaron estadísticamente por medio de: análisis de la varianza multivariada y análisis de correlación simple utilizando el paquete estadístico InfoStat. Los resultados muestran que los tiempos de deshidratación se ven influenciados por la edad del animal pero no por la posición ni por el peso del órgano.PALABRAS CLAVE: Plastinación; Acetona; Deshidratación; Riñones; Caballo. INTRODUCCIÓNLa plastinación es una auténtica revolución en el campo de la conservación de órganos reales. Gracias a esta téc-nica es posible ver, tocar y conservar órganos, piezas anató-micas y especímenes. En docencia, mejora la calidad de la enseñanza práctica y en investigación avanza en el campo de la anatomía topográfica como base para la interpretación de técnicas modernas de diagnóstico por imagen como resonancia magnética, endoscopía, tomografía computarizada, etc.Las actuales técnicas de conservación a base de formol que se utilizan en el área de anatomía de muchas universidades no son las más adecuadas. Estas presentan desventajas, como por ejemplo, la rigidez de las piezas formuladas y la pérdida del color natural. Por otro lado, las propiedades físico-químicas del formol lo convierten en un gas, el cuál afecta a las personas que lo manipulan, irritando su mucosa ocular. Su inhalación produce irritación a la mucosa nasal y al tracto respiratorio superior e incluso puede afectar los pulmones. En tiempo de exposición prolongada irrita los tejidos cutáneos (Ballenguer, 1984;Olsen et al., 1984).La técnica, desarrollada por Gunther von Hagens en Heidelberg, Alemania, se fundamenta principalmente en la sustitución de los líquidos tisulares (agua y lípidos) por polímeros (silicona o resina epóxica) y consta básicamente de cuatro etapas: fijación: luego de realizada la disección el especimen u órgano se fija en una solución de formol al 5% por un período de una semana; deshidratación, este proceso se realiza con un solvente orgánico (acetona) y tiene por finalidad la extracción del agua tisular; impregnación forzada: aquí el solvente es reemplazado por un polímero en una cámara de vacío a bajas temperaturas; curado: en esta etapa * Anatomía Animal, Depto. de Anatomía Anima...
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