BackgroundPediatric traumatic brain injuries (TBIs) have not been well studied in China. This study investigated characteristics and trends of hospitalized TBIs sustained by Chinese children.Methods and FindingsWe analyzed 2002–2011 hospitalized TBI patients (0–17 years of age) treated at a large pediatric medical center in China. TBIs were defined using the International Classification of Diseases, Tenth Revision (ICD-10) codes. We examined age patterns across external causes of TBIs. We reported the trend of traffic-related TBIs for each year from 2002 to 2011. Of 4,230 TBI patients, 67.1% (95% CI: 65.4%–68.8%) were city residents and 28.8% (95% CI: 26.3%–31.3%) came from rural villages. Males had disproportionately more TBIs than females (65.2% vs. 34.8%). Falls, struck by/against objects, and traffic collisions were the top three external causes of TBIs for all age groups. Falls were the leading cause of TBI for all ages but peaked at 2 years of age. There were 125 TBIs in 0–2 year olds (5.9% of all TBIs in this age group) that were caused by suspected child abuse. Suspected child abuse was significantly more likely to occur in 0–1 year olds. The proportion of traffic -related TBIs increased significantly from 12.99% in 2002 to 19.68% in 2008 but dropped each subsequent year until it reached a level of 8.91% in 2011.ConclusionsOur study confirms that falls, struck by/against objects and traffic collisions are the top external causes of TBIs in Chinese children. When compared with national data from the developed countries, gender patterns are similar, but the ranking of external causes is different. This is the first study to highlight the important role of suspected child abuse in causing TBIs in infants in China. TBIs caused by child abuse warrant further research and government attention as a social and medical problem in China.

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BackgroundThe injury mortality burden of Guinea has been rarely addressed. The paper aimed to report patterns of injury mortality burden in Guinea.MethodsWe retrieved the mortality data from the Guinean Annual Health Statistics Report 2007. The information about underlying cause of deaths was collected based on Guinean hospital discharge data, Hospital Mortuary and City Council Mortuary data. The causes of death are coded in the 9th International Classification of Diseases (ICD-9). Multivariate Poisson regression was used to test the impacts of sex and age on mortality rates. The statistical analyses were performed using Statatm 10.0.ResultsIn 2007, 7066 persons were reported dying of injuries in Guinea (mortality: 72.8 per 100,000 population). Transportation, fire/burn, falls, homicide and drowning were the five leading causes of fatal injuries for the whole population, accounting for 37%, 22%, 12%, 10% and 6% of total deaths, respectively. In general, age-specific injury causes displayed similar patterns of the whole population except that poisoning replaced falls as a leading cause among children under five years old. Males were at 30-50% more risk of dying from six commonest causes than females and old age groups had higher injury mortality rates than younger age groups.ConclusionTransportation, fire/burn, falls, homicide, and drowning accounted for the majority of total injury mortality burden in Guinea. Males and old adults were high-risk population of fatal injuries and should be targeted by injury prevention. Lots of work is needed to improve weak capacities for injury control in order to reduce the injury mortality burden.

There is inconsistent evidence of associations between socioeconomic status (SES) and lung cancer stage in non‐Chinese populations up to now. We set out to determine how SES affects stage at diagnosis at both individual and area levels, from a hospital‐based multicenter 10‐year (2005–2014) retrospective clinical epidemiological study of 7184 primary lung cancer patients in mainland China. Individual‐level SES data were measured based on two indicators from case report forms of the study: an individual's education and occupation. Seven census indicator variables were used as surrogates for the area‐level SES with principal component analysis (PCA). Multivariate analysis was undertaken using binary logistic regressions and multinomial logit model to describe the association and explore the effect across tertiles on stage after adjusting for demographic variables. There was a significant stepwise gradient of effect across different stages in the highest tertile of area‐level SES, comparing with the lowest tertile of area‐level SES (ORs, 0.77, 0.67, and 0.29 for stage II, III, and IV). Patients with higher education were less likely to have stage IV lung cancer, comparing with the illiterate group (ORs, 0.52, 0.63, 0.71, 0.64 for primary school, middle school, high school, college degree or above subgroup, respectively). Findings suggest that the most socioeconomically deprived areas may be associated with a higher risk of advanced‐stage lung cancer, and increasing educational level may be correlated with a lower risk to be diagnosed at advanced stage in both men and women.

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Successful eradication of H. pylori by the combination of antibiotics lowers the subsequent risk of gastric cancer and peptic ulcer disease. 1,2 However, due to the widespread use of antibiotics, antibiotic resistance and emergence of drug-resistance bacteria have become one of the largest global health threats. 3 In particular, increasing antibiotic resistance to clarithromycin has reduced the effectiveness of standard clarithromycin-based triple therapy (STT) in H. pylori eradication therapy. 4 Hence, bismuth quadruple or non-bismuth

Background Tuberculosis (TB) poses a severe public health challenge in China and worldwide. This study evaluated the effects of age, period, and birth cohort on reported incidence trends of TB based on population and refined the characteristics of high-risk groups. Methods Aggregate data that reported pulmonary tuberculosis (PTB) cases from China Tuberculosis Management Information System (TBIMS) from 2006 to 2020 were used to analyze effect coefficients through the age–period–cohort (APC) model based on intrinsic estimator (IE) method, and converted them into relative risk (RR) to estimate trends. Results A total of 14.82 million cases of PTB were reported in China from 2006 to 2020, showing a continuous downward trend. The reporting rate increased with age by age group, with 70–74 years old being 2–3 times higher than that in 20–24 years old. APC analysis model showed that age effects were bimodal in 20–24 years old [RR = 2.29, 95% confidence interval (CI): 1.73–3.03] and 70–74 years old (RR = 1.95, 95% CI: 1.67–2.27), and lower than the overall average in the groups under 15 years old. Stratified results showed that the risk was higher for women under age 40 than men and higher for men over 40. The risk was higher in urban than in rural areas under 30 years old and slightly higher in rural than in urban between 30 and 64 years old. The risk for 15–34 years old was significantly higher in the east than in other regions. The period effects showed a decreasing trend, and the risk was higher in rural in recent years. Except for cohorts born in 1961–1965 and 2001–2005, where the RR increased, the later the cohort was born, the lower the risk. The cohort 1926–1930 in eastern had the highest risk (RR = 3.49, 95% CI: 2.44–4.98). Conclusions The reported incidence of PTB continued to decline in China from 2006 to 2020. The young (20–24 years old) and the elderly (70–74 years old) were equally at high risk. There were differences in the age, period and cohort effects on PTB incidence among gender, urban–rural and regions. Our findings better reflected the characteristics of high-risk populations, thus contributing to the development of timely and effective intervention strategies, and providing clues for etiological research. Graphical abstract

The 3′ untranslated regions (UTRs), located at the end of mRNA molecules, are believed to play a role in RNA replication and/or protein translation. Mutations in the tyrosinase (Tyr) gene are known to cause recessive albinism in humans and other species. In this study, to test whether the CRISPR/Cas9 system works on the mutation of the UTRs regulatory region in rabbit, the 3′ UTR of the rabbit Tyr gene was deleted by a dual sgRNA directed CRISPR/Cas9 system. As expected, gray coat color and reduced melanin in hair follicles and irises was found in the mutated rabbit, thus increasing confidence in the association of the mutation of the Tyr 3′ UTR with graying in rabbit. The graying phenotype was also found in the F1 generation, suggesting that the mutated allele can be stably inherited by the offspring. Thus, we provide the first evidence that reduced melanin and graying can be caused by deletion of the Tyr 3′ UTR in rabbits. Additionally, CRISPR/Cas9-mediated large fragment deletions can facilitate genotype to phenotype studies of UTRs or non-coding RNAs in future.Tyrosinases (Tyr) are essential enzymes in melanin biosynthesis and are responsible for pigmentation of skin and hair in mammals 1 . Mutations in the Tyr gene result in white coat color and lack of pigmentation due to absence of melanin production. Melanin biosynthesis in these mutants is disrupted at the critical first and second reactions: hydroxylation of tyrosine to L-DOPA and the oxidation of L-DOPA to DOPA-quinone 1 . Previous studies have reported that albinismis associated with a Tyr mutation in American mink 2 , cattle 3 , mouse 4 and rabbit 5 . In an updated list of Tyr mutations, oculocutaneous albinism was also reported in humans 1 .The rabbit tyrosinase gene (Chromosome 1: 127,562,667,237) has alength of 136 kb and is composed of five exons. In albino rabbits, a homozygous mutation (T373K) was identified that resulted in alteration of the last N-glycosylation site of the Tyr coding sequence 5 . Furthermore, the CRISPR/Cas9-mediated Tyr knockout rabbit also displayed typical albinism phenotype 6, 7 .It is known that mammalian 5′ UTRs, which are non-coding DNA regulatory regions, mediate post-or co-transcriptional autoregulation of gene expression through direct interaction with proteins 8 . On the other hand, the 3′ UTRs direct an extensive range of alternative post-transcriptional, including regulation of mRNA decay and translation 9 , contributing significantly to gene regulation. It is well established that 3′ poly(A) tail of eukaryotic mRNAs is critical for the proper regulation of gene expression, and enhances the in vivo and in vitro translation in mammas 10 . In addition, several studies have highlighted the polyadenylation and alternative polyadenylation are important in gene expression and associated with a risk of clinical diseases [11][12][13][14][15] . However, all the studies were carried out in vitro or clinical cases, with very little studies conducted at the animal level. Therefore, there is a need to further characterize t...