Vulvar lichen sclerosus (VLS) is a chronic inflammatory skin disease that brings life-long and psychological distress to patients. It remains unclear whether this condition is related to changes in the skin microbial community. The aim of this study was to evaluate the compositional characteristics of the vulvar skin microbiota between VLS patients and asymptomatic postmenopausal women. We included 60 cases of postmenopausal patients in the outpatient vulvar clinic of Peking University First Hospital from August 2020 to October 2020. Thirty-one patients were diagnosed with VLS by vulvar skin biopsy (VLS group), while 29 women were asymptomatic volunteers (control group). DNA was extracted from vulvar skin swabs of the VLS and control groups. The V3-V4 fragments of 16S rRNA were targeted for high-throughput sequencing and gene sequence analysis. The sequencing results were analysed by α diversity, β diversity, species composition, LEfSe analysis to compare the compositional differences of the vulvar skin microbiota between the two groups. Our study revealed that at the phylum level, patients with VLS had a lower relative abundance of Firmicutes (p < 0.0001) and a higher relative abundance of Proteobacteria than the control group (p < 0.0001). At the genus level, Lactobacillus spp. accounted for the largest proportion of the microflora in the asymptomatic controls, while the proportion of Prevotella spp. in the VLS group was the highest. In the VLS group, the relative abundance of Finegoldia spp., Ralstonia spp., Peptoniphilus spp., Anaerococcus spp., Campylobacter spp., Providencia spp. Kelbsiella spp., Ezakiella spp., and Escherichia-Shigella spp. was significantly increased compared with the control group. Although there was no significant difference in the α diversity of the vulvar skin microbiota, the β diversity differed significantly between the two groups.
Introduction To evaluate the pelvic floor muscle function (PFMF) of cervical cancer patients after type QM-C hysterectomy and to explore the relationship between decreased PFMF and related factors..Methods This was a multi-centered retrospective cohort study. 181 cervical cancer patients who underwent type QM-C hysterectomy were enrolled from 9 tertiary hospitals. Strength of PFMF were measured by using neuromuscular apparatus (Phenix U8, French). Risk factors contributing to decreased PFMF were analyzed by univariate and multivariate ordinal ploytomous logistic regression. Results Totally 181 patients were investigated in this study. 0-3 level of type I muscle fibre strength(MFSI) was 52.6% (95/181), 0-3 level of type ⅡA muscle fibre strength(MFSⅡA) was 50% (91/181). Subjective stress urinary incontinence was 46%(84/181), urinary retention was 27.3% (50/181), dyschezia was 41.5% (75/181), fecal incontinence was 9%(18/181). ① MFSI:Multivariate ordinal ploytomous logistic regression shows that the follow-up time(p<0.05), chemotherapy and radiotherapy (p=0.038) are independent risk factors of MFSI’s reduction after type QM-C hysterectomy. ② MFSⅡA:Multivariate ordinal ploytomous logistic regression shows that the follow-up time (p<0.05) are independent risk factors of MFSⅡA’s reduction after type QM-C hysterectomy. The pelvic floor muscle strength (PFMS) increased after 9 months than in 9 months after operation, which showed that the PFMS could be recovered after operation. Conclusions We advocate for more attention and emphasis on the PFMF of Chinese female patients with cervical cancer postoperation.Contribution of the Paper PFMF after QM-C hysterectomy has not been analyzed by current study. The contribution is that patients with radical hysterectomy should do pelvic floor rehabilitation exercises in 3 months after operation.
Introduction and Hypothesis To evaluate the pelvic floor muscle function (PFMF) of cervical cancer patients after type QM-C hysterectomy and to explore the relationship between decreased PFMF and related factors.. Methods This was a multi-centered retrospective cohort study. 181 cervical cancer patients underwent type QM-C hysterectomy were enrolled from 9 tertiary hospitals. Strength of PFMF were measured by using neuromuscular apparatus (Phenix U8, French). Risk factors contributed to decreased PFMF was analyzed by univariate and multivariate ordinal ploytomous logistic regression. Results Totally 181 patients were investigated in this study.0-3 level of type I muscle fibre strength(MFSI) was 52.6%(95/181),0-3 level of type ⅡA muscle fibre strength(MFSⅡA) was 50%(91/181). Subjective stress urinary incontinence was 46%(84/181),urinary retention was 27.3%(50/181),dyschezia was 41.5%(75/181),fecal incontinence was 9%(18/181).①MFSI:Multivariate ordinal ploytomous logistic regression shows that the follow-up time(p<0.05),chemotherapy and radiotherapy (p=0.038) are independent risk factors of MFSI’s reduction after type QM-C hysterectomy.② MFSⅡA:Multivariate ordinal ploytomous logistic regression shows that the follow-up time(p<0.05) are independent risk factors of MFSⅡA’s reduction after type QM-C hysterectomy. The pelvic floor muscle strength(PFMS) increased after 9 months than in 9 months after operation, which showed that the PFMS could be recovered after operation. Conclusions We advocate for more attention and emphasis on the PFMF of Chinese female patients with cervical cancer postoperation. Contribution of the Paper The key messages of the article is that PFMF after QM-C hysterectomy have not been analyzed by current study.The new knowledge added by this study is that 3 months after radical hysterectomy patients’ should do pelvic floor rehabilitation exercises.
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