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This paper presents the Gompertz function of per capita GDP and vehicle stock to forecast the vehicle ownership of China through to 2050 against a background of increasing energy use and CO 2 emissions associated with the potential demands of on-road vehicles. We forecast the level of vehicle stock in China based on the extant patterns of vehicle development in Organisation for Economic Co-operation and Development (OECD) countries, Europe, the United States and Japan. The results show that the OECD pattern and European pattern are more suitable for describing China's vehicle stock growth when compared with Japanese and U.S. patterns. The study finds that China's vehicle stock has developed as an S-shaped curve. During the forecast period, the inflection point of the increasing curve appears around the year 2030, with the annual growth of vehicle ownership increasing from 6.13% to 9.50% in the prior period prior and subsequently dropping to 0.45% in 2050. Based on the sensitivity analysis and robustness check, the impact of different Gompertz curve parameters and GDP growth rates on vehicle stock projection are analyzed.

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We report an ultrasensitive and colorimetric assay for Cu(II) via enzymatic amplification strategy. The enzymatic activity of horseradish peroxidase (HRP) is strongly inhibited by Cu(I), which can be used indirectly to assay Cu(II). The limit of detection (LOD) is 0.37 nM, and the detection of 20 nM Cu(II) in solution can be achieved with naked eyes. This assay can be used to construct a colorimetric logic gate.

Protein kinase A (PRKACA) has been documented as a pivotal regulator in meiosis and mitosis arrest. Although our previous work has established that PRKACA regulates cell cycle progression of mouse fertilized eggs by inhibiting M-phase promoting factor (MPF), little is known about the intermediate factor between PRKACA and MPF in the mitotic cell cycle. In this study, we investigated the role of the PRKACA/CDC25B pathway on the early development of mouse fertilized eggs. Overexpression of unphosphorylatable CDC25B mutant (Cdc25b-S321A or Cdc25b-S229A/S321A) rapidly caused G2-phase eggs to enter mitosis. Microinjection of either Cdc25b-WT or Cdc25b-S229A mRNA also promoted G2/M transition, but much less efficiently than Cdc25b-S321A and Cdc25b-S229A/S321A. Moreover, mouse fertilized eggs overrode the G2 arrest by microinjection of either Cdc25b-S321A or Cdc25b-S229A/S321A mRNA, which efficiently resulted in MPF activation by directly dephosphorylating CDC2A-Tyr15, despite culture under conditions that maintained exogenous dibutyryl cAMP. Using a highly specific antibody against phospho-Ser321 of CDC25B in Western blotting, we showed that CDC25B-Ser321 was phosphorylated at the G1 and S phases, whereas Ser321 was dephosphorylated at the G2 and M phases in vivo. Our findings identify CDC25B as a potential target of PRKACA and show that PRKACA regulates G2/M transition by phosphorylating CDC25B-Ser321 but not CDC25B-Ser229 on the first mitotic division of mouse fertilized eggs.

Burning agricultural straw before and/or after harvest is a common farming practice. Regional and extensive agricultural open field straw burning can cause serious air pollution events. This paper looks at the effects of biomass burning emission on regional haze that should be considered in the forecasting of regional haze. It describes the current state of crop residue burning in China, and analyzes the relationship between biomass burning and regional haze in terms of temporal/spatial patterns and chemical composition. Finally, some suggestions/recommendations are proposed for the recycling of agricultural straw to reduce the impact of biomass burning on regional haze and air quality. We suggest that prescribed open burning would be a more suitable solution in China. We hope that this report about biomass burning and regional haze will bring the issue to the attention of governments and other researchers.

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