a b s t r a c tFirst-year students in transition from hometown to campus are generally confronted by intellectual and social challenges as well as disruption and the formation of the place attachments associated with relocation. Understanding the variables affecting student place attachment helps address the widespread concern about student transition. Interactions between place attachment to hometown and campus, and the effects of endogenous and exogenous variables on place attachments, were analysed using covariance analysis, based on questionnaire data gathered at a Chinese university. Campus identity acts as a predictor for the other three dimensions of place attachment to hometown and campus: hometown identity, hometown dependence and campus dependence. Place attachment to campus exerts a direct effect on place attachment to hometown, while the latter indirectly impacts on the former through mediators including academic self-efficacy and peer relationships. Gender, household registration record and duration of dormitory stay were also identified as statistically significant predictors of student place attachment.
A white ear lobe chicken embryo (WELCE) fibroblast cell bank, containing 322 tubes of frozen cells, was successfully established from primary explants of 57 embryo samples. The cells were morphologically consistent with fibroblasts, and the growth curve was sigmoidal with a population doubling time (PDT) of 48 h. Karyotyping and G-banding indicated a total chromosome number of 2n=78; the rate of diploidy in the cell bank was 97.62%. The cells were also free from bacterial, fungal, viral and mycoplasma contamination. Analysis of lactate dehydrogenase (LDH) and malate dehydrogenase (MDH) isoenzymes ruled out cross-contamination between cells. In order to study exogenous gene expression, six fluorescent proteins were transfected into the WELCE cells. The transfection efficiency of these genes was between 10.1 and 41.9%. The corresponding fluorescence was distributed throughout the cytoplasm and nucleus 24h after transfection. The results indicate that the quality of the cell line meet the quality requirements of the ATCC (American Type Culture Collection).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.