Angiosarcoma is a rare disease with a poor prognosis; significantly, patients with intestinal angiosarcomas who survive over 1 year after diagnosis are extraordinarily rare. This article describes the case of a 33-year-old gentleman who presented with abdominal pain of 4 months duration, which had increased in severity 2 weeks prior to presentation. After a complicated diagnostic and therapeutic process, the diagnosis of primary angiosarcoma of the small intestine with metastasis to the liver was made by pathological and immunohistochemical examinations. We reviewed previous cases of angiosarcoma described in the English literature to determine their risk factors, diagnosis and treatment, and we found that angiosarcoma is extremely rare, especially in the small intestine. To the best of our knowledge, this may be the youngest case of primary angiosarcoma of the small intestine with metastasis to the liver reported in the English literature.
N, N-Dimethylformamide (DMF) can cause liver damage in occupationally exposed workers, but the molecular mechanism of DMF-induced liver damage has not been fully elucidated. Researches have proved that lncRNA plays a major function in chemical-induced liver toxicity and can be used as a biomarker and therapeutic target for liver injury. In order to verify that lncRNA also participates in DMF-induced liver damage, we treated HL-7702 cells with 75 or 150 mM DMF, and obtained lncRNA expression profiles through high-throughput sequencing. Among the differentially expressed lncRNAs, lncRNA SNHG12 was proved to be significantly downregulated in DMF-treated HL-7702 cells and participate in DMF-mediated apoptosis, even under long-term low-dose DMF exposure (5–10 mM, 8 weeks). In addition, according to bioinformatics analysis, miR-218-5p is expected to be a potential target of SNHG12, which was verified by the dual luciferase reporter assay in HEK293FT cells. MiR-218-5p mimic can induce apoptosis in HL-7702 cells. Among the predicted targets of miR-218-5p, protein kinase C epsilon (PRKCE) was reported to be involved in apoptosis, and was indeed downregulated by miR-218-5p mimic in our study. Further experiments showed that changes of the expression of SNHG12 can affect the expression of PRKCE. In the epidemiological study of occupational population, we also found that SNHG12 was downregulated in the serum exosomes of workers exposed to DMF. These results indicated that SNHG12 can mediate DMF-induced apoptosis of HL-7702 cells through miR-218-5p/PRKCE pathway.
Shuwei Decoction itself has many functions, not only good for human body, but also has many biological functions. For functional dyspepsia rats, the distribution of connexin 43 protein and the repair and regeneration of interstitial cells of Cajal(RRICC) have different changes in
different treatment methods. The purpose of this paper is to study the effect of Shuwei Decoction on the distribution of connexin 43 protein and the RRICC in rats with functional dyspepsia. In order to prove the specific effect of Shuwei Decoction on rats with functional dyspepsia, 50 rats
were selected as the research object, and the size of distribution area of Cx43 protein and the cell regeneration were observed. The speed of birth and repair, the distribution of Cx43 protein and the level of Cx43 protein were detected, and the statistical data were studied. The results show
that Shuwei decoction can promote the regeneration and formation of ICC, thus obtaining the structural integrity of ICC, improving the potential intestinal disorder and treating FD. Shuwei decoction has a strong effect on the RRICC, which is the result of the increase of the number of intercellular
stem cells, and the speed of cell repair and regeneration is increased by nearly 30%. On the basis of the distribution of Cx43 protein, the distribution area increased by 20% compared with the original basis. Therefore, Shuwei decoction has a great influence on the distribution of connexin
43 proteins and the RRICC in rats with functional dyspepsia.
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