[1] Antigorite, the high-temperature (HT) form of serpentinite, is believed to play a critical role in various geological processes of subduction zones. We have measured P-and Swave velocities (V p and V s ), anisotropy and shear-wave splitting of 17 serpentinite samples containing >90% antigorite at pressures up to 650 MPa. The new results, combined with data for low-temperature (LT) lizardite and/or chrysolite, reveal distinct effects of LT and HT serpentinization on the seismic properties of mantle rocks. At 600 MPa, V p = 5.10 and 6.68 km/s, V s = 2.32 and 3.67 km/s, and V p /V s = 2.15 and 1.81 for pure LT and HT serpentinites, respectively. Above the crack-closure pressure (~150 MPa), the velocity ratio of antigorite serpentinites displays little dependence on pressure or temperature. Serpentine contents within subduction zones and forearc mantle wedges where temperature is >300 C should be at least twice that of previous estimates based on LT serpentinization. The presence of seismic anisotropy, high-pressure fluids, or partial melt is also needed to interpret HT serpentinized mantle with V p < 6.68 km/s, V s < 3.67 km/s, and V p /V s > 1.81. The intrinsic anisotropy of the serpentinites (3.8-16.9% with an average value of 10.5% for V p , and 3.6-18.3% with an average value of 10.4% for V s ) is caused by dislocation creepinduced lattice-preferred orientation of antigorite. Three distinct patterns of seismic anisotropy correspond to three types of antigorite fabrics (S-, L-, and LS-tectonites) formed by three categories of strain geometry (i.e., coaxial flattening, coaxial constriction, and simple shear), respectively. Our results are thought to provide a new explanation for various anisotropic patterns of subduction systems observed worldwide.Citation: Ji, S., A. Li, Q. Wang, C. Long, H. Wang, D. Marcotte, and M. Salisbury (2013), Seismic velocities, anisotropy, and shear-wave splitting of antigorite serpentinites and tectonic implications for subduction zones,

This paper provides results of an experimental investigation of three hybrid drying technologies on drying characteristics and the key quality parameters of shiitake mushroom (Lentinus edodes). The drying techniques tested at laboratory scale are mid-infrared assisted convection drying (MIRCD), hot air coupled with radio frequency drying (HCRFD) and hot air coupled with microwave drying (HCMD). For comparison the standard drying technique using hot air was also tested. The quality parameters include texture, color, rehydration rate, shrinkage, nutrient retention and microstructure etc. These four drying tests were conducted at fixed air temperature (60°C), and the power level for HCRFD, MIRCD and HCMD, which was fixed at 4W/g. The resultsshowed that hot air coupled with microwave drying gave the shortest drying time, while mid-infrared assisted convection and hot air coupled with radio frequency dried products Downloaded by [RMIT University] at 06:55 25 August 2014 ACCEPTED MANUSCRIPT ACCEPTED MANUSCRIPT 2 showed the better color attribute and nutrient retention. Under conditions tested mid-infrared assisted convection drying yielded minimal shrinkage (maximal rehydration), and lower hardness upon rehydration. The uniform honeycomb network and less collapsed structure of MIRCD samples can be used to explain these better quality characteristics.

A large portion of the middle to lower crust beneath the continents and oceanic island arcs consists of amphibolites dominated by hornblende and plagioclase. We have measured P and S wave velocities (Vp and Vs) and anisotropy of 17 amphibole‐rich rock samples containing 34–80 vol % amphibole at hydrostatic pressures (P) up to 650 MPa. Combined petrophysical and geochemical analyses provide a new calibration for mean density, average major element contents, mean Vp‐P and Vs‐P coefficients, intrinsic Vp and Vs anisotropy, Poisson's ratios, the logarithmic ratio Rs/p, and elastic moduli of amphibole‐rich rocks. The Vp values decrease with increasing SiO2 and Na2O + K2O contents but increase with increasing MgO and CaO contents. The maximum (≤0.38–0.40 km/s) and minimum S wave birefringence values occur generally in the propagation direction parallel to Y and normal to foliation, respectively. Amphibole plays a critical role in the formation of seismic anisotropy, whereas the presence of plagioclase, quartz, pyroxene, and garnet diminishes the anisotropy induced by amphibole crystallographic preferred orientations (CPOs). The CPO variations cause different anisotropy patterns illustrated in the Flinn diagram of Vp(X)/Vp(Y)‐Vp(Y)/Vp(Z) plots. The results make it possible to distinguish, in terms of seismic properties, the amphibolites from other categories of lithology such as granite‐granodiorite, diorite, gabbro‐diabase, felsic gneiss, mafic gneiss, eclogite, and peridotite within the Earth's crust. Hence, amphibole, aligned by dislocation creep, anisotropic growth, or rigid‐body rotation, is the most important contributor to the seismic anisotropy of the deep crust beneath the continents and oceanic island arcs, which contains rather little phyllosilicates such as mica or chlorite.

BackgroundImpaired glucose tolerance (IGT) is a pre-diabetic state of hyperglycemia that is associated with insulin resistance, increased risk of type II diabetes, and cardiovascular pathology. Recently, investigators hypothesized that decreased vagus nerve activity may be the underlying mechanism of metabolic syndrome including obesity, elevated glucose levels, and high blood pressure.MethodsIn this pilot randomized clinical trial, we compared the efficacy of transcutaneous auricular vagus nerve stimulation (taVNS) and sham taVNS on patients with IGT. 72 participants with IGT were single-blinded and were randomly allocated by computer-generated envelope to either taVNS or sham taVNS treatment groups. In addition, 30 IGT adults were recruited as a control population and not assigned treatment so as to monitor the natural fluctuation of glucose tolerance in IGT patients. All treatments were self-administered by the patients at home after training at the hospital. Patients were instructed to fill in a patient diary booklet each day to describe any side effects after each treatment. The treatment period was 12 weeks in duration. Baseline comparison between treatment and control group showed no difference in weight, BMI, or measures of systolic blood pressure, diastolic blood pressure, fasting plasma glucose (FPG), 2-hour plasma glucose (2hPG), or glycosylated hemoglobin (HbAlc).Results100 participants completed the study and were included in data analysis. Two female patients (one in the taVNS group, one in the sham taVNS group) dropped out of the study due to stimulation-evoked dizziness. The symptoms were relieved after stopping treatment. Compared with sham taVNS, taVNS significantly reduced the two-hour glucose tolerance (F(2) = 5.79, p = 0.004). In addition, we found that taVNS significantly decreased (F(1) = 4.21, p = 0.044) systolic blood pressure over time compared with sham taVNS. Compared with the no-treatment control group, patients receiving taVNS significantly differed in measures of FPG (F(2) = 10.62, p < 0.001), 2hPG F(2) = 25.18, p < 0.001) and HbAlc (F(1) = 12.79, p = 0.001) over the course of the 12 week treatment period.ConclusionsOur study suggests that taVNS is a promising, simple, and cost-effective treatment for IGT/ pre-diabetes with only slight risk of mild side-effects.

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Aim: To investigate the neuroprotective effects of morin on 1-methyl-4-phenylpyridinium ion (MPP + )-induced apoptosis in neuronal differentiated PC12 cells as well as in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model of Parkinson disease (PD). Methods: PC12 cells were challenged with MPP + in the presence or absence of morin. Cell viability was determined using MTT assay. Cell apoptosis was measured using flow cytometry. Generation of reactive oxygen species (ROS) was assayed using fluorescence assay. In an MPTP mouse model of PD, behavioral deficits, striatal dopamine content, and number of dopaminergic neurons were measured. Results: MPP + induced apoptosis and ROS formation in PC12 cells. Concomitant treatment with morin (5-50 μmol/L) significantly attenuated the loss of cell viability and apoptosis when compared with MPP + treatment alone. Morin also attenuated ROS formation induced by MPP + . MPTP induced permanent behavioral deficits and nigrostriatal lesions in mice. When administered prior to MPTP, morin (20 to 100 mg/kg) attenuated behavioral deficits, dopaminergic neuronal death and striatal dopamine depletion in the MPTP mouse model. Conclusion: The findings suggest that morin has neuroprotective actions both in vitro and in vivo, and may provide a novel therapeutic agent for the treatment of PD and other neurodegenerative diseases.

Myotonic dystrophy type 1 (DM1) is caused by a CTG nucleotide repeat expansion within the 3′ UTR of the Dystrophia Myotonica protein kinase gene. In this study, we explored therapeutic genome editing using CRISPR/Cas9 via targeted deletion of expanded CTG repeats and targeted insertion of polyadenylation signals in the 3′ UTR upstream of the CTG repeats to eliminate toxic RNA CUG repeats. We found paired SpCas9 or SaCas9 guide RNA induced deletion of expanded CTG repeats. However, this approach incurred frequent inversion in both the mutant and normal alleles. In contrast, the insertion of polyadenylation signals in the 3′ UTR upstream of the CTG repeats eliminated toxic RNA CUG repeats, which led to phenotype reversal in differentiated neural stem cells, forebrain neurons, cardiomyocytes, and skeletal muscle myofibers. We concluded that targeted insertion of polyadenylation signals in the 3′ UTR is a viable approach to develop therapeutic genome editing for DM1.

Background Campylobacter species are the major food-borne pathogens which could cause bacterial gastroenteritis in humans. Contaminated chicken products have been recognized as the primary vehicles of Campylobacter transmission to human beings. In this study, the prevalence of Campylobacter in retail chicken meat in Central China was investigated, and the isolates were further characterized using molecular approaches and tested for antibiotic resistance.ResultsA total of 302 chicken samples purchased from April 2014 to April 2015 were tested. The level of Campylobacter contamination was enumerated by most probable number-PCR (MPN-PCR). The Campylobacter positive rate was 17.2% (52/302), with bacterial count varying from 3.6 to 360 MPN/g in positive samples. A total of 52 Campylobacter strains, including 40 Campylobacter jejuni and 12 Campylobacter coli, were isolated from the positive samples. To examine the genetic diversity of the isolates, multilocus sequence typing (MLST) technology was applied, which identified 23 sequence types (STs) belonging to seven clonal complexes (CCs) and unassigned. Among them, the dominant CCs of C. jejuni included CC-353 and CC-464, and the dominant CCs of C. coli were CC-828 and CC-1150. Antibiotic resistance analysis showed that all of the isolates were resistant to norfloxacin and ciprofloxacin. 23 virulence-associated genes were tested in the isolates, which showed that the number of virulence-associated genes detected in the C. jejuni isolates ranged from 16 to 21, while in most of the C. coli isolates ranged from 12 to 16. Virulence-associated genes, flaA, flgB, flgE2, fliM, fliY and cadF were detected in all isolates. VirB11, however, was not detected in any of the isolates.ConclusionsTo the best of our knowledge, this is the first report on the contamination level and molecular biological features of Campylobacter strains in retail chicken meat in Central China, which showed high genetic diversity and remarkable antibiotic resistance. This study provided scientific data for the risk assessment and evaluation of Campylobacter contamination in retail chicken products.Electronic supplementary materialThe online version of this article (doi:10.1186/s13099-016-0132-2) contains supplementary material, which is available to authorized users.