Understanding the causes of the Sino-Japanese disjunctions in plant taxa has been a central question in eastern Asian biogeography, with vicariance or long-distance dispersal often invoked to explain such patterns. Diabelia Landrein (Caprifoliaceae; Linnaeoideae) comprises four shrubby species with a Sino-Japanese disjunct distribution. The species diversification time within Diabelia, covering a long geological history of the formation process of the Sino-Japanese flora, dated back to the middle Oligocene, therefore, Diabelia would be an ideal model to elucidate the biogeographic patterns of Sino-Japanese disjunctions with climate fluctuation. In this study, we analyzed complete plastome sequence data for 28 individuals representing all four species of Diabelia. These 28 plastomes were found to be highly similar in overall size (156 243-157 578 bp), structure, gene order, and content. Our phylogenomic analysis of the plastomes supported a close relationship between Diabelia ionostachya (Nakai) Landrein & R.L. Barrett var. wenzhouensis (S.L. Zhou ex Landrein) Landrein from eastern China and Diabelia spathulata (Siebold & Zucc.) Landrein var. spathulata from Japan. Diabelia serrata (Siebold & Zucc.) Landrein was identified as sister to a population of Diabelia sanguinea (Makino) Landrein from Tochigi in central Japan and D. spathulata Landrein, from Toyama, central Japan. Most Diabelia lineages were estimated to have differentiated 8-28 Mya. Our results indicate that two independent vicariance events could explain the disjunction between Japan and Korea in the mid to late Miocene, and between Zhejiang and Japan in the early Miocene.
Local control of neural blood flow is considered to reside in innervation of epineurial and endoneurial arterioles rather than in intrinsic autoregulatory mechanisms. With the use of an isolated vessel preparation and an in vivo approach, the present studies examined intrinsic vasomotor responsiveness of epineurial arterioles. Segments of epineurial arterioles, cannulated on glass micropipettes (40 μm) and pressurized in the absence of intraluminal flow, showed sustained pressure-dependent (30–90 mmHg) vasoconstriction and acute myogenic reactivity. Myogenic tone was unaffected by phentolamine (10−6 M). Removal of extracellular Ca2+ resulted in loss of spontaneous tone and passive behavior. Concentration-response curves for norepinephrine (10−9–3 × 10−6 M) and relaxation to both acetylcholine (10−8–10−5M) and adenosine (10−8–10−4M) were obtained. Acetylcholine dilator responses were inhibited by N G-nitro-l-arginine methyl ester. Epineurial blood flow was measured in vivo using a laser-Doppler flow probe. Blood flow declined over a 2-h period after surgery, and during this time preparations developed responsiveness to the dilator acetylcholine. Phentolamine blocked vasoconstrictor responses to exogenous norepinephrine but only partially reversed the in vivo baseline tone. The time-dependent decline in epineurial blood flow was observed despite the presence of tetrodotoxin (1 μM), further confirming that tone was predominantly caused by myogenic rather than neurogenic mechanisms. It is concluded that because epineurial arterioles exhibit intrinsic myogenic reactivity, they have the potential to participate in local regulation of neural hemodynamics independently of their own innervation.
This paper mainly aims at the special requirement of the solar energys application in tunnel direct lighting engineering, where the light receiver is fixed. Therefore, it has given a new automatic tracking method of polar axis type, which can effectively make the concentrator to realize the function of tracking the sun automatically with high precision and fixed-point output. Based on expounding the working principle of the automatic tracker with polar axis, meanwhile combining with the obliquely installed concentrator load, it talks about the detailed design and explanation of mechanical structure, characteristics and implementation of the suns hour angle and declination angle tracking mechanism.
Alkaline pectinase has a broad application prospect in textile field. In hemp degumming, enzymatic degumming is preferred because of the better fiber quality and less environmental pollution. In this thesis, we used the recombinant strain Bacillus subtilis TCCC11485 constructed in our laboratory to express high-yield alkaline pectinase. The optimal conditions of the alkaline pectinase for hemp degumming were determined by single factor experiments as: bath ratio 1:36, pH 8, 45°C, enzyme activity 600 U/mL, degumming for 6 h. Under the above conditions, the residual gum content of fiber is 32.76%. Further, the scanning electron microscopy (SEM) was also used to compare quality of the hemp fibers produced by enzymatic-, chemical-degumming. The results showes that the fibers are in higher quality by alkaline pectinase degumming, suggesting the prospect of commercial applications in domestic textile industry.
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