BackgroundWe determined whether bone marrow mesenchymal stem cells (BMMSCs) transduced with heme oxygenase-1 (HO-1), a cytoprotective and immune-protective factor, could improve outcomes for small bowel transplantation (SBTx) in rats.MethodsWe performed heterotopic SBTx from Brown Norway rats to Lewis rats, before infusing Ad/HO-1-transduced BMMSCs (Ad/HO-1/BMMSCs) through the superficial dorsal veins of the penis. Respective infusions with Ad/BMMSCs, BMMSCs, and normal saline served as controls. The animals were sacrificed after 1, 5, 7, or 10 days. At each time point, we measured small bowel histology and apoptosis, HO-1 protein and mRNA expression, natural killer (NK) cell activity, cytokine concentrations in serum and intestinal graft, and levels of regulatory T (Treg) cells.ResultsThe saline-treated control group showed aggravated acute cellular rejection over time, with mucosal destruction, increased apoptosis, NK cell activation, and upregulation of proinflammatory and immune-related mediators. Both the Ad/BMMSC-treated group and the BMMSC-treated group exhibited attenuated acute cellular rejection at an early stage, but the effects receded 7 days after transplantation. Strikingly, the Ad/HO-1/BMMSC-treated group demonstrated significantly attenuated acute cellular rejection, reduced apoptosis and NK cell activity, and suppressed concentrations of inflammation and immune-related cytokines, and upregulated expression of anti-inflammatory cytokine mediators and increased Treg cell levels.ConclusionOur data suggest that Ad/HO-1-transduced BMMSCs have a reinforced effect on reducing acute rejection and protecting the outcome of SBTx in rats.
Plunge milling cutter withstanding greater loads in heavy milling can cause tool breakage, cutting vibration, even chatter. Firstly, geometric model of plunge milling cutter on various combinations of axial rake angle and radial rake angle are established. Secondly, with combination different axial rake angle and radial rake angle of plunge milling cutter under static load, the amount of deformation plunge milling cutter are calculated by finite element analysis. Finally, the plunge milling cutter first six natural frequencies are given in plunge milling cutter modal analysis with finite element analysis. The results show that minimum total deformation of plunge milling cutter is given with the axial rake angle 5° and radial rake angle-5 ° of the cutter and when the tool working at frequencies up to 10012HZ has caused vibration within the XY plane.
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