Previous work has shown that the reflection from aquarium walls can modify several aspects of crayfish behaviour, but only if the crayfish are socialized. Such socialization is known to generate a dominance hierarchy composed of dominant and subordinate animals. The present study was conducted to determine whether or not behavioural responses to reflection depend on dominance status. Procambarus clarkii (Girard, 1852) were maintained in pairs for 2 weeks to establish dominance ranks and were subsequently observed in an aquarium in which half of the walls were lined with mirrors and the other half were lined with nonreflective plastic. Dominant crayfish spent more time on the reflective side of the aquarium than on the nonreflective side, and they spent more time in reflective corners than in nonreflective corners. Dominant crayfish also turned more frequently on the reflective side and crossed the tank more frequently toward reflective walls. Such differences in behaviour were not observed for subordinate crayfish or for crayfish that had been isolated for 2 weeks. Subordinate crayfish exhibited more reverse walking on the reflective side of the aquarium than on the nonreflective side, but crayfish in the other groups did not. Thus, responses of crayfish to reflection depend on dominance status.

No abstract

Social behavior depends on sensory input from the visual, mechanical and olfactory systems. One important issue concerns the relative roles of each sensory modality in guiding behavior. The role of visual inputs has been examined by isolating visual stimuli from mechanical and chemosensory stimuli. In some studies (Bruski & Dunham, 1987: Delgado-Morales et al., 2004 visual inputs have been removed with blindfolds or low light intensity, and effects of remaining sensory modalities have been elucidated. An alternative approach is to study the effects of visual inputs in the absence of any appropriate mechanical and chemosensory cues. This approach aims to identify the exclusive role of visual inputs.We have used two methods to provide visual stimuli to crayfish without providing chemical and mechanical cues. In one method, crayfish are videotaped in an aquarium where half of the walls are covered in mirrors to provide a reflective environment, and the other half are covered in a non-reflective (matte finish) plastic. This gives the crayfish a choice between reflective and non-reflective environments. The reflective environment provides visual cues in the form of reflected images of the crayfish as it moves throughout half of the tank; these visual cues are missing from the non-reflective half of the tank. An alternative method is to videotape the behavior of crayfish in an aquarium separated by a smaller chamber at each end, with a crayfish in one small chamber providing visual cues and an inert object in the opposite small chamber providing visual input from a non-moving, non-crayfish source.Our published results indicate that responses of crayfish to the reflective environment depend on socialization and dominance rank. Socialized crayfish spent more time in the reflective environment and exhibited certain behaviors more frequently there than in the non-reflective environment; isolated crayfish showed no such differences. Crayfish that were housed in same-sex pairs developed a social rank of either dominant or subordinate. Responses to reflection differed between dominant and subordinate crayfish (May & Mercier, 2006; May & Mercier, 2007). Dominant crayfish spent more time on the reflective side, entered reflective corners more frequently and spent more time in reflective corners compared to the non-reflective side. Subordinate crayfish walked in reverse more often on the reflective side than on the non-reflective side. Preliminary data suggest similar effects from visual cues provided by a crayfish in a small adjoining chamber (May et al., 2008).

No abstract