1. Ecological theory indicates that warmer and more stable climates should result in stronger biotic interactions. Therefore, plant species growing at lower elevations and experiencing greater herbivore pressure should invest in higher levels of defences than those at higher elevations. Nonetheless, there are a number of studies that have found no effect of elevational gradients on plant defensive traits. Several factors might explain the lack of consistency for the altitude-defence relationships, including (i) the reduction of all defensive traits into one measure of resistance; (ii) not considering plant defence as the simultaneous expression of several defensive traits; and (iii) not considering the relative influence of biotic (e.g. herbivory) and abiotic (e.g. climate and soil conditions) factors associated with the ecological gradient.2. Here, we present a comprehensive test of the effects of elevation and its associated biotic and abiotic factors on the individual and simultaneous expression of constitutive direct and indirect defences and their inducibility (i.e. expression of defences after herbivore attack). Specifically, we estimated climatic and soil variables and measured herbivore damage and constitutive and jasmonic acid-induced glucosinolate levels in the leaves as a proxy for direct defences, and volatile emission as a proxy for indirect defences in 16 Cardamine species naturally growing along the steep elevational gradient of the Alps. 3. Within a phylogenetic comparative framework, we found that species growing at lower elevations invested more in the simultaneous inducibility of both direct and indirect defences, whereas species growing at higher elevations invested more in constitutive direct defences. Although we found strong elevational gradients in herbivory and climatic and soil variables, these biotic and abiotic factors only partially explained elevational patterns in plant defences. 4. Synthesis. These results highlight that the complex regulation of multiple defence traits strongly vary across elevational gradients and build towards a better understanding of the multiple mechanisms underlying trait evolution and species interactions along ecological gradients.
Insect herbivores trigger various biochemical changes in plants, and as a consequence, affect other organisms that are associated with these plants. Such plant-mediated indirect effects often involve herbivore-induced plant volatiles (HIPVs) that can be used as cues for foraging herbivores and their natural enemies, and are also known to affect pollinator attraction. In tightly co-evolved systems, the different trophic levels are expected to display adaptive response to changes in HIPVs caused by native herbivores. But what if a new herbivore invades such a system? Current literature suggests that exotic herbivores have the potential to affect HIPV production, and that plant responses to novel herbivores are likely to depend on phylogenetic relatedness between the invader and the native species. Here we review the different ways exotic herbivores can disrupt chemically mediated interactions between plants and the key users of HIPVs: herbivores, pollinators, and members of the third (i.e. predators and parasitoids) and fourth (i.e. hyperparasitoids) trophic levels. Current theory on insect invasions needs to consider that disruptive effects of invaders on infochemical networks can have a short-term impact on the population dynamics of native insects and plants, as well as exerting potentially negative consequences for the functioning of native ecosystems.
Root herbivores are notoriously difficult to study, as they feed hidden in the soil. However, root herbivores may be traced by analyzing specific volatile organic compounds (VOCs) that are produced by damaged roots. These VOCs not only support parasitoids in the localization of their host, but also may help scientists study belowground plant-herbivore interactions. Herbivore-induced VOCs are usually analyzed by gas-chromatography mass spectrometry (GC-MS), but with this off-line method, the gases of interest need to be preconcentrated, and destructive sampling is required to assess the level of damage to the roots. In contrast to this, proton-transfer-reaction mass spectrometry (PTR-MS) is a very sensitive on-line, non-invasive method. PTR-MS already has been successfully applied to analyze VOCs produced by aboveground (infested) plant parts. In this review, we provide a brief overview of PTR-MS and illustrate how this technology can be applied to detect specific root-herbivore induced VOCs from Brassica plants. We also specify the advantages and disadvantages of PTR-MS analyses and new technological developments to overcome their limitations.
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