Background:
CE is a zoonotic parasitic infection caused by
Echinococcus granulosus
worldwide and is associated with economic losses among livestock animals
.
EG95 is an immunogenic antigen from the
E. granulosus.
Lactococcus lactis
has been prested as a safe vehicle for antigen delivery. The goal of this study was to design a novel
L. lactis
strain displaying EG95 as a vaccine delivery system.
Methods:
The
eg
95 encoding gene fragment fused to the M6 anchoring protein was cloned into the pNZ7021 vector, and
L. lactis
NZ9000 displaying recombinant EG95 was constructed. The expression of an approximately 32-kDa EG95 protein was confirmed by Western blotting and immunofluorescence analysis. The immune responses were evaluated in BALB/c mice immunized orally and subcutaneously with the live and killed recombinant
L. lactis
, respectively.
Results:
Total IgG level in mice immunized with heat-killed recombinant
L. lactis
(pNZ7021-
eg
95) significantly increased compared to the control group. sIgA was significantly higher in mice received live recombinant
L. lactis
(pNZ7021-
eg
95) compared to the control mice. Splenic lymphocytes from immunized mice represented the high levels of IFN-γ and the low-levels of IL-4 and IL-10.
Conclusion:
Our results indicate that immunization with EG95-expressing
L. lactis
can induce both specific humoral and cellular immune responses in mice.
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