The process of enzyme assisted extraction of essential oil from the leaves and branches of the Vietnamese aromatic plant Cinnamomum cassia was studied and optimized using a Box-Wilson central composite design consisting of 05 independent variables (pH, temperature T, time τ, concentration of the enzyme Laccase, and concentration of the enzyme Cellic Htec2) and two dependent variables (reducing sugar and yield of essential oil). Second-order polynomial equations were obtained for the responses, which fitted well with the experimental data. Optimal conditions for oil yield were found at pH = 5.2; T = 440C; τ = 5h30'; Laccase = 0.42 ml/g, and Cellic Htec2 = 1.15%. The experimental value (0.982% oil yield) was close to the predicted value (0.978%). The application of enzyme assisted extraction in combination with optimization using response surface methodology substantially improved the oil yield as compared with traditional method.
In order to improve the efficiency of essential oil distillation of the leaves and branches from the cassia plant (Cinnamomum cassia (L.) J. Presl) growing in Yen Bai province, the effects of enzyme treatments of the plant materials before distillation were investigated using crude laccase obtained from culture medium of the fungus Ganoderma lucidum and Cellic Htech 2 (Novozymes, Denmark), a multienzyme system consisting of cellulase and xylanase. The results showed that enzyme treatments increased oil yield and shorten distillation time. The use of a mixture of both enzyme systems was more effective than using them separately. Under optimal conditions, an increase in oil yield of 41.7 % was achieved, while distillation time was shortened from 8 to 5 hours. The enzyme treatments did not change the qualitative composition of the essential oils. However, significant changes in the percentages of cinnamic aldehyde (69.74 % to 85.6 %) and cinnamyl acetate (17.2 % to 1.34 %) were observed.
This study presents a chemometric study on agarwood (Aquilaria crassna) essential oils extracted from selected agarwood samples grown in various regions of Asia. Adopting gas chromatography-mass spectrometry (GC-MS) technique, it was revealed that essential oils, produced by hydrodistillation,constitutes mainly volatile aromatic compounds. Several major components are shared in all samples including dihydro-agarofuran-15-al, jinkoeremol, 10-epi-γ-eudesmol, agarospirol, valerianol, n-hexadecanoic acid, neopetasane and dihydrokaranone. Despite differences in composition, extraction yield and detected constituents found in analyzed samples, characteristic aromatic compounds were abundantly found in the Agarwood essential oil. These discrepancies could be due to cultivation season, climatic conditions and extraction methods. Unambiguous identification of components in agarwood essential oils thereby opens new potential in the application of high-value aromatic compounds in agarwood essential oil in cosmetic products, perfumes, and pharmaceuticals.
The essential oils (EOs) of the aerial parts of four Asarum species (A. geophilum, A. yentunensis, A. splendens and A. cordifolium) were isolated by steam distillation and analyzed by the GC/MS method. The A. cordifolium EO contains 33 constituents with the main component being elemicine (77.20%). The A. geophilum EO was contains 49 constituents with the main components being determined as 9-epi-(E)-caryophyllene (18.43%), eudesm-7(11)-en-4-ol (13.41%), β-caryophyllene (8.05%) and phytol (7.23%). The A. yentunensis EO contains 26 constituents with the main components being safrole (64.74%) and sesquicineole (15.34%). The EO of A. splendens contains 41 constituents with the main components being 9-epi-(E)-caryophyllene (15.76%), eudesm-7(11)-en-4-ol (14.21%), β-caryophyllene (9.52%) and trans-bicyclogermacrene (7.50%). For antimicrobial activity, the A. yentunensis EO exhibited the highest inhibition activity against Staphylococcus aureus and the A. cordifolium EO against Bacillus subtillis (MIC values of 100 μg/mL). For antioxidant activity, the A. geophilum EO showed the highest potential with an SC (%) value of 63.34 ± 1.0%, corresponding to an SC50 value of 28.57 µg/mL. For anti-inflammatory activity, the A. splendens EO exhibited the highest potential with an IC50 value of 21.68 µg/mL, corresponding to an inhibition rate of NO production of 69.58 ± 1.3% and the percentage of cell life was 81.85 ± 0.9%.
Agarwood from Aquilaria crassna cultivated in Khanh Hoa province was extracted by 5 methods, including simple, enzyme-assited, and microwave-assisted hydrodistillation, extraction with solvent and supercritical carbon dioxide. The volatile compounds of the products were analyzed by GC-MS. Almost all samples contain some common characteristic components, such as Valerianol, Agarospirol, Eudesmol <b->, Eudesmol <10-epi-g->, 2-Allyl-4-methylphenol, Guaiol and Neopetasone. However, there were obvious differences in yields, composition, and number of identified components. The application of the more advanced methods for the extraction of agarwood resulted in saving of time and energy, and some improvement of product yield. However, these methods also lead to unconventional products with many unknown components, what requires further investigations.
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