High-performance liquid chromatography with postcolumn fluorescence derivatization for the simultaneous determination of glycated albumin and D-glucose in human serum is described. Glycated albumin and D-glucose were separated along with other glycated proteins on a size exclusion column (TSKgel G2000SWXL) and converted into fluorescence derivatives by reaction with 4-methoxybenzamidine or meso-l,2-bis(4-methoxyphenyl)ethylenediamine in an alkaline medium. The method required only 1 µl of serum. The detection limits for glycated albumin and D-glucose (pmol on column) were 100 and 3 with the former reagent, and 150 and 5 with the latter, respectively.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.