Pemphigus, a group of autoimmune blistering dermatoses recognized in humans and dogs, is divided into three subgroups: pemphigus vulgaris (PV), pemphigus foliaceus (PF) and paraneoplastic pemphigus (PNP). Previously, we have demonstrated that circulating anti-desmoglein 3 (Dsg3) IgG in dogs with PV and PNP disrupts Dsg3-mediated keratinocyte cell-cell adhesion. The aim of this study was to develop an enzyme-linked immunosorbent assay (ELISA) for the detection of circulating IgG against canine Dsg3 in dogs with pemphigus. A secreted form of recombinant protein representing the entire extracellular domain of canine Dsg3 (cDsg3) was produced by baculovirus expression and used as the coated antigen in the ELISA. The titre of anti-cDsg3 ELISA was significantly higher in canine PV (n = 14) than that in healthy dogs (n = 44) (P < 0.05). Of the canine PV and PNP sera, 11/14 (79%) canine PV sera and the canine PNP serum were positive in the anti-cDsg3 ELISA. On the other hand, 14/37 (38%) sera from canine PF and 9/18 (50%) sera from canine autoimmune subepidermal bullous dermatoses were positive in the ELISA. Circulating IgG against cDsg3 was not recognized in dogs with superficial pyoderma (n = 5), atopic dermatitis (n = 8), or in healthy dogs (n = 44). Moreover, IgG immunoreactivity against cDsg3 in all 21 sera was completely absorbed by preincubation with cDsg3. These findings altogether suggest that the anti-cDsg3 ELISA can be used as a rapid screening test for the detection of circulating anti-Dsg3 IgG autoantibodies in canine pemphigus.
Factors relating with the etiology and clinical courses after treatment of calcinosis cutis in 8 dogs with Cushing's disease were analysed retrospectively. Calcinosis cutis occured in 8 out of 16 dogs with Cushing's disease. The occurrence of calcinosis cutis was suggested to be correlated with the high plasma cortisol concentration measured at the first admission. Clinical course was followed in 6 cases and the temporary exacerbation of the calcificated skin lesion in four of the six cases was observed in the process of improvement.
Vaccination is being promoted around the world as an effective countermeasure against COVID -19. The Oku -Aizu Homecare Center (OAHC) , a division of the Fukushima Prefectural Miyashita Hospital, has provided support for group vaccination in the three Oku -Aizu towns of Yanaizu, Mishima, Kaneyama, and the village of Showa. The OAHC began discussing vaccination with the three Oku -Aizu towns and the village in March 2021 and vaccinations began in May 2021. Between May and August 2021, 20 group vaccination sessions were delivered, with the OAHC providing support for all
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