A simple micellar liquid chromatographic method was developed and validated for the determination of azosemide (AZS). The method incorporates surfactant media beyond the critical micellar concentration level for the study of AZS in bulk and pharmaceutical dosage forms. Quantitation was achieved through UV detection. An interpretive optimization procedure was used to ascertain the optimum conditions based on the accurate description of the retention and shape of the chromatographic peak for quantification of AZS, viz., selection of surfactants, pH of buffer solution, concentration of surfactant and selection of solvent etc. The method was successfully validated as per the requirements of the international Council for Harmonisation (ICH) guidelines and showed excellent linearity within the limits of detection and limit of quantitation. The method was linear over the concentration range 0.001–0.2 mg ml−1 with regression coefficient (r2) 0.999. The proposed method was successfully applied for ascertaining the stability and determination of AZS under different stressed conditions viz., basic, acidic, oxidative, photolytic, and thermal degradation. The method elicited good selectivity, repeatability, linearity and sensitivity of the validated parameters. Recoveries obtained were in a range of 98.90–99.58%. The method allows accurate and reliable determination of AZS for drug stability assays in pharmaceutical investigations using environmentally friendly materials.
In the original publication of the article, the corresponding author's name was incorrectly published. The corresponding author's name should appear as Ashok Kumar.
A high performance liquid chromatography method, using micellar solution above the critical micellar concentration (CMC) has been developed and validated to quantify baclofen (a muscle relaxant drug) in active pharmaceutical ingredients and pharmaceutical formulations. Process optimization was done at various conditions such as different surfactants, surfactant concentration, pH of buffer solution, solvent composition, solvent selection and different HPLC columns. An isocratic HPLC mode, column temperature 35 °C, Intersil ODS-3 V 250 × 4.6, 5 µm column, 0.8 mL/min mobile phase flow rate and UV detection wavelength at 220 nm was used for the purpose. The method was validated as per International Conference on Harmonization (ICH) guidelines viz., specificity, linearity, range, inter and intra-day precision, accuracy (recovery) and robustness. Forced degradation conditions such as basic, acidic, oxidative, thermal degradation and photolytic are applied to prove the stability indicating nature of the proposed method.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.