A new basic peptide antibiotic designated as K-582 was isolated, purified and characterized. When K-582 was applied to a column of A1203 or Bio-Gel P-2 or CM Sephadex, two major peaks which were named Fraction I (K-582 A) and Fraction II (K-582 B) were obtained. The nitrogen content, the behavior in color reaction, the absorption bands of amide linkages in the infrared absorption spectrum, 1H NMR spectrum and C-13 NMR spectrum indicated the peptide nature of K-582 A and K-582 B. K-582 was effective against yeasts, but inactive against other Gram-positive bacteria, Gram-negative bacteria and Mycobacterium. The toxicity was low in mice.During our screening program for substances with antibiotic activity, we found a new substance named K-582 produced by the strain 582M which was isolated from a soil sample collected in Sendai and from the results of detailed taxonomical studies the strain was identified as Metarhizium anisopliae (METSCH.) SOROK. Var. anisopliae.The antibiotic, K-582, exhibited a significant growth inhibition of Candida in liquid media, of viruses in tissue culture and of ascites tumor in mice.This paper deals with the characterization of Metarhizium anisopliae (METSCH.) SOROK. var. anisopliae 582 M, the fermentation process, the isolation procedure and the properties of K-582. Taxonomical StudiesStrain 582 M was isolated by SHIGEJI KONDO from a soil sample collected in the vicinity of Sendai City, Miyagi Prefecture, Japan. The taxonomical characterization was carried out according to the methods described by BARNETT1), BARRON2) and TULLOCH3) and the strain 582 M was classified as Metarhizium anisopliae (METSCH.) SOROK. var. anisopliae. Macroscopic descriptionColonies on CZAPEK-peptone agar grew rapidly, attaining a diameter of 4.5 -5.0 cm in 2 weeks at 28°C. The colonies are more or less fasciculated, plane or sometimes lightly furrowed radially. They have white margins with olivaceous to dark olivaceous colored sporulating areas in center. Colonies are often intermixed or zonate. Transformation to light yellow to dark olivaceous colors occurs. Exudate is lacking. The odor was conspicuously pungent similar to that of streptomyces (Tables 1, 2 and 3.).
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.