BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

No abstract

Aequorea green fluorescent protein (GFP) and protein A were fused and expressed in Escherichia coil The fluorescent native fusion protein (PA-GFP) migrated at 47 kDa in SDS-PAGE. However, the non-fluorescent denatured PA-GFP migrated at 57 kDa which corresponds to the theoretical molecular mass. Although the reason(s) for this mobility shift between fluorescent and non-fluorescent molecules remains unclear, the small ring structure within the native molecules may affect their mobility. The cell extract, prepared from an E. coli strain producing PA-GFP, was used in Western and dot blots. The sensitivity and specificity of the PA-GFP detection were sufficient for rapid and easy screening.

No abstract

-Rimicaris kairei new species (Alvinocarididae: Caridea: Decapoda) is described based on materials, from the active hydrothermal vent field, "Kairei Field," on the Central Indian Ridge, the Indian Ocean. The new species is clearly distinguishable from the unique congener, R. exoculata Williams & Rona, 1986, which is known from the Atlantic Ocean. The following morphological differences were identified: (1) all punctations on carapace strongly ornamented by tufts of short stiff setae in R. exoculata , whereas without any setae in R. kairei ; (2) antennal flagella in R. exoculata shorter than in R. kairei ; (3) walking legs in R. exoculata more robust than in R. kairei .