Objective The Agu is the only native pig breed in Japan, which is reared in Okinawa prefecture, the southernmost region in Japan. Its origins are considered to be of Asian lineage; however, the genetic background of the Agu is still unclear. The objective of this study was to elucidate the maternal lineage of the Okinawa indigenous Agu pig with the use of the mitochondrial DNA (mtDNA) control region. Methods The mtDNA control regions of Agu pigs were sequenced and the phylogenetic relationship among Agu, East Asian and European pigs was investigated with the use of 78 Agu individuals. Results Twenty-seven polymorphic sites and five different haplotypes (type 1 to type 5) were identified within the Agu population. Phylogenetic analysis indicated that types 1 and 2 were included in East Asian lineages; however, the remaining types 3, 4, and 5 were of European lineages, which showed a gene flow from European pigs in the 20th century. Sixty-seven out of 78 Agu individuals (85.9%) possessed mtDNA haplotypes 1 and 2 of the East Asian lineage, which were identical to two haplotypes of ancient mtDNA (7,200 to 1,700 years before the present) excavated at archaeological sites in Okinawa. Conclusion This study confirmed that the East Asian lineage is dominant in the maternal genetic background of the Agu population, supporting the hypothesis that the ancestors of the Agu pig were introduced from the Asian continent.
The effect of providing a diet containing a large quantity of linoleic acid (C 18: 2n-6) on the production of cow's milk rich in conjugated linoleic acid was examined using four lactating Holstein cows (mean milk yield: 26.8kg).One group of cows was fed with experimental diet containing linseed and the other group was fed with diet without linseed for a period of 56 days. The control and experimental cows received daily 224g of C 18: 2n-6/head and 308g of C 18: 2n-6/head, respectively. A mean cis-9, trans-11/traps-9, cis-11-octadecadienoic acid (CLA-1) content of the milk fat of the total fatty acids for the control and the experimental cows, respectively. On the other hand, mean trans-11-octadecenoic acid contents of the milk fat and serum lipid increased from 2.2 to 5.4% and from 0.93 to 2.48%, respectively, as a result of feeding the experimental diet.
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