When considering drug delivery, the amount of drug that can be carried at a particular time and how the drug is incorporated efficiently into cells are important parameters. Transferrin (Tf)-conjugated nanocarriers have been used for the targeted delivery of drugs to cancer cells due to the availability of receptor-mediated clathrin-dependent endocytosis. In general, however, endocytosis seems to differ according to the size and shape of carriers. Large substances are generally internalized into cells by phagocytosis. We studied the internalization mechanism of Tf-conjugated nanoparticles (diameter, 522 nm). Tf-conjugated polystyrene particles were incorporated into cells by receptor-mediated endocytosis with large clathrin-coated vesicles even though their diameter was >500 nm and despite that fact that clathrin-coated vesicles have a diameter of ≈100 nm. This finding suggests that signals for internalization generated by stimulated Tf receptors (TfRs) activate clathrin-mediated endocytosis preferentially. Whether these larger particles could deliver drugs more efficiently than smaller particles was then examined. The toxicity of larger Tf-conjugated biodegradable nanoparticles (poly(lactic-co-glycolic acid)) encapsulating doxorubicin (diameter, 216 ± 38 nm) was appreciably dependent on the number of Tf molecules conjugated on a particle and the number of TfRs expressed on the cell membrane. Larger Tf-conjugated particles delivered drugs to cancer cells expressing many TfRs more selectively than their smaller counterparts (diameter, 56 ± 9 nm) if they were decorated with an appropriate number of Tf molecules.
Usukura (2020) A method to selectively internalise submicrometer boron carbide particles into cancer cells using surface transferrin conjugation for developing a new boron neutron capture therapy agent,
This article highlights the issues of exposure inhomogeneity that are relative to eye lens monitoring for low-energy photons from 241Am and beta-rays from 90Sr/90Y including a personal protective equipment because eye lens exposure has been concerned more than before due to the proposed reduction of relevant dose limit. These nuclides are common and concerned sources in the nuclear industry. Our previous study presented a quantitative estimation of exposure inhomogeneity, which was applied to simple but typical exposure situations. For the present study, exposure inhomogeneity of 241Am was approximately within a factor of 1.6, implying a more homogeneous situation than expected. Regarding 90Sr/90Y exposure, estimation from both Hp(10) and Hp(0.07) on trunk would lead to an over- or underestimation by a factor of more than 10. In contrast, Hp(3) measurement on trunk will improve by up to a factor of 2. With respect to the personal protective equipment, lead apron and protective glasses are effective for the 60-keV photons for both anterior-posterior and rotational irradiations, while a full-face respirator can reduce the eye lens dose by approximately 17% for 90Sr/90Y betas. As a whole, this study demonstrated that the effect of protective equipment could be effectively incorporated into the homogeneity evaluation.
We demonstrate a practical calibration method and its applicability for a commercially available radiophotoluminescence dosemeter (RPLD), i.e. the GD-352M (AGC Techno Glass, Shizuoka, Japan) to eye lens dose monitoring, by performing the calibration according to the ISO recommendations. The calibration was then verified through a series of experiments. For verification of the derived calibration factor (1.21 ± 0.04, k = 1) of the RPLD, we performed standard irradiations in the ISO narrow series X-ray reference fields and the simulation measurements in the actual radiation fields in a hospital. The TLD-based commercially available dosemeters, DOSIRIS™ was also put on the ISO cylinder phantom and the RANDO phantom together with the GD-352M in the verification experiments. The personal dose equivalents Hp(3) obtained from the GD-352M and those obtained from the DOSIRIS™ were in good agreement with each other. Our results demonstrate the proper calibration of a commercially available RPLD that is applicable to the additional monitoring of the lens of the eyes for medical staff.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.