Background: Japan is one of the few countries believed to have eliminated soil-transmitted helminths (STHs). In 1949, the national prevalence of Ascaris lumbricoides was 62.9%, which decreased to 0.6% in 1973 due to improvements in infrastructure, socioeconomic status, and the implementation of national STH control measures. The Parasitosis Prevention Law ended in 1994 and population-level screening ceased in Japan; therefore, current transmission status of STH in Japan is not well characterized. Sporadic cases of STH infections continue to be reported, raising the possibility of a larger-scale recrudescence of STH infections. Given that traditional microscopic detection methods are not sensitive to low-intensity STH infections, we conducted targeted prevalence surveys using sensitive PCR-based assays to evaluate the current STH-transmission status and to describe epidemiological characteristics of areas of Japan believed to have achieved historical elimination of STHs.Methods: Stool samples were collected from 682 preschool-and school-aged children from six localities of Japan with previously high prevalence of STH. Caregivers of participants completed a questionnaire to ascertain access to water, sanitation and hygiene (WASH), and potential exposures to environmental contamination. For fecal testing, multi-parallel real-time PCR assays were used to detect infections of Ascaris lumbricoides, Necator americanus, Ancylostoma duodenale and Trichuris trichiura.Results: Among the 682 children, no positive samples were identified, and participants reported high standards of WASH.
Conclusions:To our knowledge, this is the first STH-surveillance study in Japan to use sensitive molecular techniques for STH detection. The results suggest that recrudescence of STH infections has not occurred, and that declines in prevalence have been sustained in the sampled areas. These findings suggest that reductions in prevalence below the elimination thresholds, suggestive of transmission interruption, are possible. Additionally, this study provides circumstantial evidence that multi-parallel real-time PCR methods are applicable for evaluating elimination status in areas where STH prevalence is extremely low.
We reported 865 cases of soil-transmitted nematodiasis occurring in Japan during 2000-2017. The predominant nematode was Strongyloides stercoralis (n = 279, 32.3% of all cases), and other species included Ascaris lumbricoides (30.7%), Trichuris trichiura (23.1%), and Ancylostomidae spp. (13.9%). Strongyloides stercoralis was detected primarily in patients in Okinawa and Kagoshima prefectures, which are in the south of Japan and are endemic areas for this parasitic infection, and also in about half of the prefectures of all Japan. At least 15.5 cases of strongyloidiasis occurred on average each year. The period incidence rate of strongyloidiasis cases relative to the total population of Japan was 0.012 cases per 10 5 person-years. The male-to-female ratio was 2.1. The average age was 75.1 ± 16.9 years, and 96.1% of patients were older than 50 years. Several reasons may explain why this previously non-endemic outside of Okinawa region, serious nematode disease is now found in much of Japan, including the increased number of transmigration and sightseeing trips in Japan, use of immunosuppressive drugs, and lack of awareness of the risks. Thus, information of strongyloidiasis and its risks must be disseminated to travelers, residents, and physicians to prevent this life-threatening parasite infection.
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