Purpose: To establish a simple and accurate photodynamic diagnosis (PDD) method for oral squamous cell carcinoma (OSCC). Methods: OSCC cell lines HSC-2, HSC-3, HSC-4, and Sa3, and normal human oral keratinocytes (HOK) were used. First, we examined the amount of cells needed to detect differences in fluorescence intensities for PDD. OSCC cell lines were adjusted to concentrations of 1 × 10 4 (10 4 ), 1 × 10 5 (10 5 ), and 1 × 10 6 (10 6 ) cells/ml. The experimental groups comprised a group with 5-aminolevulinic acid (5-ALA (+)), and a group without 5-ALA (5-ALA (−)). For each OSCC cell line, 100 µl of each concentration of cells of the 5-ALA groups was seeded onto fluorescence plates, and fluorescence intensity was measured at 60-min intervals for 240 min. Results are expressed as the ratio of fluorescence intensity in 5-ALA (+) to 5-ALA (−). As cells at the concentration of 10 6 cells/ml provided the clearest results, fluorescence intensities of all cell lines were measured using this concentration at 20-min intervals for 700 min using the same methods. Results: The 5-ALA (+) to (−) ratio increased in a cell concentration-dependent manner at 240 min; the ratio was highest with 10 6 cells/ml and lowest with 10 4 cells/ml. With 10 6 cells/ml in the 5-ALA (+) group, fluorescence intensity increased in a metabolic time-dependent manner; the increase was highest in HSC-2 cells, followed by HSC-4 cells, HSC-3 cells, Sa3 cells, and HOK. Fluorescence intensity was significantly enhanced after 40 min in HSC-2, HSC-3, and HSC-4 cells, after 60 min in Sa3 cells, and after 100 min in HOK compared to the 5-ALA (−) group (P < 0.05). Moreover, fluorescence intensity was significantly increased in OSCC cell lines compared to HOK after 40 min. Conclusion: Early detection of OSCC is possible How to cite this paper: Omori, H. and Komine, C. (2021) Development of a Photodynamic Diagnosis Method for Oral Squamous Cell Carcinoma Using 5-Aminolevulinic Acid and a Luminescence Plate Reader.
Background and aim : The aims of the present study were to investigate the relationship between 1 O 2 generated via rutile-TiO 2 and its bactericidal effect on oropathogenic microorganisms, and to evaluate whether antimicrobial photodynamic therapy (a-PDT) via rutile-TiO 2 is applicable to dental treatment. Materials and methods : The experimental groups were defined as follows: only physiological saline without lightemitting diode (LED) irradiation; control group, physiological saline with LED irradiation; LED group, physiological saline including TiO 2 (4 mg/ mL) with LED irradiation; and the photocatalyst (PC) group. The LED irradiation time periods were set to 1, 2 and 3 min. A control group was placed without irradiation at room temperature. Reactive oxygen species and the amount of 1 O 2 generated were measured. A representative bacterial species selected from each oral infectious disease was used for bactericidal testing, including Streptococcus mutans (S. mutans) , Enterococcus faecalis (E. faecalis) , Porphyromonas gingivalis (P. gingivalis) , Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) , and Tannerella forsythia (T. forsythia). The colonyforming units per milliliter (CFU/mL) present after incubation was examined by experimental group, and the relative relationships between the amount of 1 O 2 generated and the various sterilization rates were examined. Results : Whereas S. mutans and E. faecalis were hardly sterilized in all groups, there were remarkable bactericidal effects in the LED group and PC group for A. actinomycetemcomitans, T. forsythia, and P. gingivalis. Conclusion : It appears that, by controlling the amount of 1 O 2 , one can select the bactericidal effect, and theses findings contribute to the development of an evidencebased medicine approach to a-PDT.
CoV -2 infection. On the other hand, serological diagnostic methods using immunochromatography, which can detect virusspecific antibodies in serum in a shorter time than with PCR, are becoming popular. In this study, a SARS -CoV -2specific IgG antibody test using immunochromatography was performed on 52 people who worked at the Nihon University Hospital, School of Dentistry at Matsudo (NUHSDM) . No SARS -CoV -2specific IgG antibodypositive individuals were found. In addition to expanding the inspection system for SARS -CoV -2 and examining further infection prevention measures, establishing measures to prevent further infection spread when an infected person is identified is urgently necessary.
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