Abstract-The serum levels of immunoreactive gastrin (IRG) and secretion of gastric juice were simultaneously determined in dogs anesthetized with morphine and urethane. There was a significant positive linear correlation between secretion and serum IRG level in these dogs. Serum IRG level and gastric secretion were reduced by bilateral vagotomy at the neck. The amount of gastric juice was reduced dose-dependently by an intravenous injection of atropine (0.001-0.016 mg/kg), hexamethoniurn (0.064-1 mg/kg) and secretin (2-8 U/kg). The reduction of gastric secretion paralleled that of the serum IRG level. However, the reduction of gastric secretion did not parallel that of serum IRG level under the influence of prostaglandin E1 (0.002-0.008 mg/kg i.v.) and duodenal acidification.Prostaglandin E1 and duodenal acidification reduced gastric secretion without the reducing serum IRG level. These findings were discussed in relation to the mechanism of gastric juice stimulation by morphine, and it is suggested that endogenous gastrin release through the vagal and non-vagal pathways participates in morphine-induced gastric secretion. The difference in inhibitory effect between duodenal acidification and secretin suggests the possibility that substances other than secretin may participate in the regulation of gastric secretion in dogs.
Abstract-Relationship between serum gastrin levels and gastric secretion was studied in Heidenhain pouch dogs. Bethanecol and tetramethylammonium increased gastric secretion without any significant change in the serum immunoreactive gastrin (IRG) level. Histamine increased gastric secretion but decreased the serum IRG level. Tetragastrin evoked gastric secretion concomitantly with an elevation of the serum IRG level, and the relationship was significant.Food-intake promptly increased the serum IRG level which correlated with the increased gastric secretion. Except for the first 15-min value after food-intake, a better correlation was obtained and was almost the same as that with tetragastrin-stimulation.Hexamethonium reduced the foodinduced secretion rate in parallel with reduction of the serum IRG level. Correlation between the secretion rate and serum IRG level after dosing was almost the same as that of the control. Atropine and secretin induced a stronger inhibition on the secretion rate than on the serum IRG level. Prostaglandin E/reduced the secretion rate, but produced no inhibitory effect on the serum IRG level. These results suggest that the food-induced gastric secretion in Heidenhain pouch dogs is due to the action of en dogenous gastrin, and that hexamethonium and prostaglandin E1 affect respectively the gastrin and parietal cells. Atropine and secretin affect both parietal and gastrin cells.
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