Cleome viscose Linn. also known as the jakhya are widely utilised in traditional and ethnomedicine. Biosynthesis of calcium oxide nanoparticles has captured attention of many as due synthesis involve non-toxic and eco-friendly solvents and ingredients, is more environmentally friendly, least time taking and cost-effective, and simpler than the other alternatives. In the study CaCO3 was obtained from conch shell. CaONPs were biosynthesized in methanolic extract of Cleome viscosa leaves through precipitation and deposition of CaCO3. The synthesized CaO nanoparticle was having the average particle size of ~72 nm according to DLS and the particle was found to be stable with zeta potential of -21.6 mV. The SEM analysis of nanoparticle predicted the structure to be roughly round. The UV-Visible spectrophotometer analysis predicted the maximum absorption in the visible range of ~400-420 nm. The synthesized CaO nanoparticle was found to be quite effective against BT-474 breast cancer cell line of conc. 3.4 mg/ml having cell cytotoxicity of ~ 86% at this concentration and IC50 of nanoparticle was 1.359 mg/ml. The IC50 of Antioxidative assay was 282 µg/ml and 525 µg/ml for DPPH and ABTS free radicals respectively.

Cleome viscosa, often known as Jakhya, is a common weed that may be found in farms, gardens, and a variety of other areas. Because of its significant anti-disease effect, the plant and its parts have been employed in traditional medicine. Aim: Current study aim to purify the bioactive having potent antioxidant activity and anticancer activity against BT-474 cell lines. Methods: Cleome viscosa leaf methanolic extracts were purified through preparative HPLC and the isolated compounds were checked against MCF10A (normal cell line) and BT-474 (breast cancer cell line). Results: HPLC isolated components from methanolic leaves extract included atropine, nevirapine, gallic acid, caffeic acid, vanillic acid, and kaempferitrin. Compounds were more efficient in terms of DPPH. Kaempferitrin inhibited DPPH by 94%. Atropine, vanillic acid, and kaempferitrin were shown to be cytotoxic to BT474 cell lines, with IC50 values of 18.87, 1.316, and 46.42 µg/ml, respectively. Incubating with their IC50 concentrations caused no morphological harm to the cell. Only the number of cells decreased significantly as incubation time increased. Conclusion: Cleome viscosa purified compounds showed good antioxidant and anticancer activity. The compounds were found to be effective against BT474 breast cancer cell line.

The annual, sticky plant Cleome viscose Linn. (Capparidaceae), sometimes known as "Jakhya," thrives as a weed across India's plains and throughout the tropics. The entire plant and its components are extensively used in traditional and folklore medicine (seeds, leaves, roots and barks). We evaluated the antimicrobial and antioxidant capacity of Cleome viscose leaf extract.Methods: Methanol was used to extract the leaves. Extract was analysed qualitatively and quantitatively. Antioxidative properties of extract was assayed using DPPH, FRAP, ABTS H2O2 scavenging assay. Correlation between antioxidant activity, phenolic content and flavonoid content was estimated. Antimicrobial activity of Cleome viscose Linn leaf methanolic extract was estimated against Staphylococcus aureus, Vibrio cholera, Salmonella Typhimurium.Result: The methanolic extract of Cleome viscose leaves showed good antioxidant and antimicrobial potential. The extract was having alkaloids, phenolics, flavonoids and tannins as major phytochemicals. The extract showed good ABTS scavenging activity and moderate DPPH scavenging activity. The TPC strongly while TFC moderately correlated with antioxidant activity of the extract.Conclusion: Methanolic extract of Cleome viscose leaves demonstrated significant antioxidant and antibacterial activity. The phytoconstituents found in plant leaves, primarily phenolics, flavonoids, alkaloids, and tannins, are thought to be responsible for the plant's antioxidant and antimicrobial properties, making it a therapeutically important plant with the potential to be used as a drug as an antioxidant or against microbial diseases.