Alzheimer’s disease (AD) has been associated with the hallmark features of cholinergic dysfunction, amyloid beta (Aβ) aggregation and impaired synaptic transmission, which makes the associated proteins, such as β-site amyloid precursor protein cleaving enzyme 1 (BACE I), acetylcholine esterase (AChE) and synapsin I, II and III, major targets for therapeutic intervention. The present study investigated the therapeutic potential of three major phytochemicals of Rosmarinus officinalis, ursolic acid (UA), rosmarinic acid (RA) and carnosic acid (CA), based on their binding affinity with AD-associated proteins. Detailed docking studies were conducted using AutoDock vina followed by molecular dynamic (MD) simulations using Amber 20. The docking analysis of the selected molecules showed the binding energies of their interaction with the target proteins, while MD simulations comprising root mean square deviation (RMSD), root mean square fluctuation (RMSF) and molecular mechanics/generalized born surface area (MM/GBSA) binding free energy calculations were carried out to check the stability of bound complexes. The drug likeness and the pharmacokinetic properties of the selected molecules were also checked through the Lipinski filter and ADMETSAR analysis. All these bioactive compounds demonstrated strong binding affinity with AChE, BACE1 and synapsin I, II and III. The results showed UA and RA to be potential inhibitors of AChE and BACE1, exhibiting binding energies comparable to those of donepezil, used as a positive control. The drug likeness and pharmacokinetic properties of these compounds also demonstrated drug-like characteristics, indicating the need for further in vitro and in vivo investigations to ascertain their therapeutic potential for AD.
Background: People nowadays have developed a new passion of weightlifting. Weightlifting focuses on vigorous muscle development. But injuries are also common in weightlifting. This study aims to compare the injury rates among supervised and non-supervised weightlifters. Methods: A group of 138 weight lifters was divided into two groups i.e. who did training under supervision and the other who did training without any supervision. Injuries related to musculoskeletal system were identified using Nordic musculoskeletal questionnaire. Data was analyzed using SPSS. Chi square test was used to see the association of musculoskeletal pain among weightlifters with or without supervision. Results: Significant association found between musculoskeletal injuries and supervision. Injuries lesser in number among supervised weightlifters as compared to unsupervised weightlifters. Mean age of weight lifters under supervision and without supervision was 21.99 (SD 3.81) and 24.64 (SD 5.01) respectively. Mean workout days /week among weight lifters under supervision was almost same i.e. 5.67 (SD .63) and was 5.62 (SD .81). Out of 51 participants who work-out for 46-60 min, 30 were not under supervision while 31 weightlifters who work-out for 61-90 min were working out under supervision. Injury rate was more in the region of shoulder in both groups supervised and unsupervised groups while hip/thigh region was less involved in both supervised and unsupervised groups. Conclusion: Overall results showed significant association between musculoskeletal injuries and supervision. Injury rate was more among weightlifters who work without supervision as compared to those who work under supervision. Care should be taken and weight lifting and exercises must be performed under expert’s supervision.
The disability which occurs frequently in children is known as cerebral palsy (CP). Its prevalence in Pakistan is much higher and about 2 to 3 out of every 1000 children are diagnosed with CP, which affectstheirfunctional status of life to a greater extent. The objective of the current study was to determine the association of CP child's behavior with caregiver's stress level. The current study was based on a cross sectional design and itcomprised 159 caregivers of CP child. Among them 100 were males and 59 were females. The study was conducted within the time period from December 2016 to May 2017. A convenience sampling technique was used and subjects were selected from different hospitals and rehabilitation centers of Lahore, Pakistan on the basis of inclusion and exclusion criteria after taking informed consent. The two standardized “parental stress scale” and “strength difficulty questionnaire” helped to determine the level of stress and caregiver' burden. The data was analyzed using SPSS v20. The frequency was calculated to obtain mean values of caregiver stress and behavior of CP child using PSS and SDQ. P value ≤ .05 was considered statistically significant. The Pearson correlation (r) between PSS and SDQ data was r₌ 0.63, which showed a strong positive linear correlation between the two variables, that is, CP child behavior & caregiver stress level. It indicated that both variables increased or decreased together. The behavior of CP child affects the quality of life of parents and caregivers. The study concluded that there is a strong relationship between the CP child's behavior with the caregiver’s stress level.
Background: Stroke is clinically diagnosed as an acute, localized neurological impairment that is caused by vascular damage (infarction, hemorrhage) to the central nervous system. This main leading cause of demise and dysfunction worldwide resulting in frequent deficit, hemiplegia, which briefly affects upper limb. This multicentered study is the first to provide updated normative reference values for FMA-UE in post stroke adults in wide range of age in Pakistan’s population. Objective: To determine Pakistani normative values of Fugl-Meyer Assessment on upper extremity in post Stroke Population. Methods: Descriptive cross-sectional study on 1260 post-stroke patients was conducted in various public and private physiotherapy centers of country using Fugl-Meyer Assessment (Upper-Extremity) FMA-UE scale after ethical approval. Hemiplegic survivors of both genders aged 45-75 years were taken using convenience sampling technique, informed consent was taken. Statistics was done using SPSS version25. Results: Patients included were 88.3% ischemic and 11.7% hemorrhagic. 44.1% were in acute phase (less than 2 weeks) while 55.9% were in sub-acute (2 weeks to 6 months) phase. Mean score of FMA-UE was 88.63±30.09. The cutoff score/mean value for motor impairment and sensation was recorded as 41.49 ±19.7 and 8.6±3.9 while passive joint motion and pain were calculated 18.93±5.47 and 19.57±5.1 respectively. Conclusion: The scoring, in acute and subacute phases, accentuate the need to follow Pakistani specific modified norms in Asian inhabitants instead of reference values applicable on western population. Keywords: Fugl-Meyer scale, Hemiplegia, Motor control, Post Stroke, Upper extremity.
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