(4) 1,0 mg/l de ANA+3,0 mg/l de 6-BAP+2 mg/l de ácido giberélico (AG3). El enraizamiento se realizó ex vitro se plantaron en materas en un medio preparado con tierra de hormiga, arena fina y musgo en proporciones iguales (1:1:1), desinfestadas con formaldehido un mes antes del iniciado el proceso. Resultados: A los 90 días, el número de brotes y tamaño de los brote por explantes fue: cultivar 1 C1 (78,4±05) brotes y longitud del brote (2,5±0,03 cm); cultivar 2 C2: (85,7±0,5) brotes y longitud del brote (2,1±0,11 cm), Cultivar3 C3 (92,8±0,1) brotes y longitud del brote (1,6±0,07 cm). La mejor combinación de los reguladores de crecimiento fue de 1 mg/l de ANA y 3 mg/l de 6-BAP, lo cual indica que es necesaria la presencia de auxina y citoquinia para la brotación. El enraizamiento fue ex vitro y a los 90 días las plantas tenían en promedio de C1 (5,8±0,4) raíces y longitud de raíz (3,2±0,3 cm); C2 (5,4±0,1) raíces y longitud de raíz (3,9±0,8 cm); C3 (5,7±0,7) raíces y longitud de raíz (3,8±0,5 cm).Palabras clave: Cultivares, Cultivo in vitro, Micropropagación, Piña, Propagación masiva. AbstractObjective: Massively propagate pineapple three cultivars of Chocó in Colombia, Ananas comosus (L. Merr.). Methodology: in vitro multiplied buds of apices extracted from crown and basal children cultured in semisolid Murashige and Skoog initiation medium (MS, 1962) containing: 4 mg. l-1 thiamine HCl, 0,5 mg/l NAA, 0,5 mg/l IBA and 0,5 mg/l 6-BAP and pH adjusted to 5,8; 10 g/l activated charcoal, and then a multiplication medium with four hormone treatments; (1) 0,3 mg/l of naphthalene acetic acid (NAA)+1,0 mg/l of benzylaminopurine (6-BAP), (2) 0,5 mg/L NAA+1,5 mg/l BAP, (3) 1,0 mg/l NAA+3,0 mg/l 6-BAP, (4) 1,0 mg/l NAA + 3,0 mg/l 6-BAP+2 mg/l of gibberellic acid (GA3). Rooting ex vitro was performed were planted in pots in a medium prepared with soil from ant, fine sand and moss in equal proportions (1:1:1), disinfested with formaldehyde a month before the process starts. Results: At 90 days, the number of shoots and shoots size of explants was: C1 (78,4±0,5) shoots and shoots length (2,5±0,03 cm); C2: (85,7±0,5) shoots and shoots length (2,1±0,11 cm), C3 (92,8±0,1) shoots and shoots length (1,6±0,07 cm). The best combination of growth regulators was 1 mg/l NAA and 3 mg/l de 6-BAP.lo indicating that the presence of auxin and citoquinia necessary for sprouting. The rooting was ex vitro, and at 90 days the plants had an average of: C1 (5,8±0,4) roots and root length (3,2±0,3 cm); C2 (5,4±0,1) roots and roots length (3,9±0,8 cm); C3 (5,7±0,7) roots and roots length (3,8±0,5 cm).
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