We synthesized 7-substituted-3-(2,4-dinitrostyryl)cephalosporin derivatives which were Nitrocefin analogs, for detecting extended spectrum beta-lactamase (ESBL) specifically. HMRZ-86 which has carboxypropyloxyimino group on 7-aminothiazolacetamide substituent were not hydrolyzed by class A, C and D beta-lactamases, but it was hydrolyzed by ESBL and metallo beta-lactamase (class B), then its color changed from yellow to red. The hydrolysis of metallo beta-lactamase was inhibited by adding sodium mercapto acetic acid (SMA). Therefore HMRZ-86 is a useful chromogenic agent to detect ESBL specifically.
A New Synthesis of a Functionalized Bicyclo(5.4.0)undecane Skeleton Based on a Sequence Involving (2 + 2) Photoaddition and Regioselective β-Scission of Alkoxyl Radicals Generated from the Resulting Cyclobutanols.-The present method can be similarly applied to the construction of 5/7, 5/8, and 6/8 bicyclic systems.-(SUGINOME, H.; NAKAYAMA, Y.; HARADA, H.; HACHIRO, H.; ORITO, K.; J. Chem. Soc., Chem. Commun. (1994) 4, 451-452; Org. Synth. Div., Fac. Eng., Hokkaido Univ., Sapporo 060, Japan; EN)
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