The findings suggest that C allele carriers of the SELE gene polymorphism (A561C) might be predisposed to EH in the Chinese population. Further investigations in other ethnic populations should be conducted to verify these findings.
This paper presents an integral strength checking method to solve the problem that the step-by-step strength checking method cannot check the strength of metal parts in the rubber mounting system of light truck powertrain. By establishing an integral finite element model including powertrain and rubber mount system and considering the rubber bearing and force transfer mode, the motion coordination and overall analysis of mount system and powertrain are realized. The calculation results show that the proposed method can easily and accurately complete the strength check of metal parts of the mounting system, which provides a reference for the structural design of the mounting system.
Tetrodotoxin (TTX) is a deadly neurotoxin and usually accumulates in large amounts in the ovaries but is non-toxic or low toxic in the testis of pufferfish. The molecular mechanism underlying sexual dimorphism of TTX accumulation in gonads is complex and unclear. Piwi/piRNA complexes are essential for germline specification, gametogenesis, and gonadal development, they also demonstrate sexual dimorphism in teleosts. Hence, the present study investigated the expression of piRNAs and mRNAs by transcriptomics in cultured pufferfish Takifugu flavidus after intramuscular administration of exogenous TTX. The results showed 80 piRNAs were down-regulated and 223 genes were up-regulated in the ovary after TTX administration. By contrast, 286 piRNAs were down-regulated and 445 genes were up-regulated after TTX administration in testis. Functional and pathway analyses indicated that the TTX up-regulated genes were enriched in the Wnt, ErbB and GnRH signaling pathways in ovary, while were enriched in the oocyte meiosis, estrogenesis biosynthesis and cell apoptosis-related pathways in testis. Interestingly, these genes were also the potential target genes of TTX downregulated piRNAs. Amhr2 and cyp19a were also identified as sex-related genes, involved in TTX administration. These results showed a certain level of consistency with the enrichment pathway analysis, which indicated that the TTX could affect the expression of sex-related genes, and work as an inhibitor for the testicular meiosis, but as a promoting factor for ovary development through piRNAs in pufferfish. In addition, TUNEL staining showed that significant apoptosis was detected in the TTX treated testis, and the role of the cell apoptotic pathway was further confirmed. Overall, this research will contribute to an understanding of the piwi function in TTX sexual dimorphism accumulation in pufferfish and provide a basis for further studies.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.