Objective: The combination between the stem bark powder of Chirabilva and Veerataru is used ethno folklore for the management of anticonvulsion by tribal people. To standardise the evidently used polyherbal formulation in a systemic way for supporting the identity, quality, purity, safety and efficacy concern with modern technique. Methods:Microscopic anatomical examination and powder microscopy were performed fresh and dried plant materials respectively. The compound polyherbal formulation is processed by organoleptic characterization, macro-microscopic evaluation, physicochemical, phytochemical testing, DNA fingerprinting and high-performance thin-layer chromatography (HPTLC) profiling employing a standard methodology. Chromatographic fingerprinting after visualisation the data are exploited by multivariate chemometric technique. Results:Results of the experiment provided diagnostic characteristics to identify quality and purity and standardise the polyherbal formulation along with respective ingredients. The RAPD analysis of Dichrostachys cinerea and Holoptelea integrin folia showed some similar bands at the same base pair indicate the presence of genetical identity may take into consideration of the control the ingredients. HPTLC technique utilised to distinguish the ingredient and polyherbal formulation based on the presence or absence of certain target phytochemical (flavonoid, polyphenol, etc.) constituents, manifested as peaks or bands from the chemical fingerprint profiles. Visualized chromatographic profile of polyherbal formulation along with its constituents applying the multivariate chemometric technique is easily discriminated of respective retardation factor in principal component score space. Conclusion:The findings from this study will provide systemic evaluation for this anticonvulsant formulation and also serve as a master document to control the quality of polyherbal formulation.
Background: Kwatha preparation is one among the Pnachvidha Kashaya Kalpana. The ratio of water and hardness of drug plays an important role during preparation of Kwatha. Disparities in proportions of liquid (water) to be mentioned can be observed in the classics, which are based on the quality (the hardness) and quantity of the raw material being used in the pharmaceutical procedure. Aims and objective: The objective of the present study was to evaluate the significance of proportion of water in the preparation of Kashaya and Ghana. The water proportion was fixed as 4 parts, 8 parts and 16 parts. Material and methods: Three batches of Kashaya and Ghana with each proportion were prepared considering the batch size as 1 kg and findings were systematically recorded. Organoleptic evaluation, Physico-chemical parameters of Kwatha and Ghana, HPTLC and HPLC were carried out. Result and discussion: Average time took for 1/4th reduction was found to be 339 min, 570 min and 960 min and in the preparation of Kashaya with 4 parts, 8 parts and 16 parts of water respectively while Average temperature maintained during the process was 95-1000 C. An average of 6.23%, 6.24% and 6.32% Ghana was obtained from the Kashaya in 4,8,16 parts of water respectively. The change in organoleptic parameters, physico - chemical parameters, HPTLC & HPLC study was observed. The physico-chemical parameters of all the samples presented negligible differences. After spray, 7 spots were observed in the sample prepared with 4 parts of water and remaining samples provided only 5 spots. In HPLC study, the batch 4, 8 and 16 have almost similar concentrations. Conclusion: Though the percentage of Ghana is similar in all the batches; during the pharmaceutical procedure, it is advisable to add 8 parts of water. This facilitates proper soaking and boiling of the raw material.
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